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Flotillin-1 Regulates IgE Receptor-Mediated Signaling in Rat Basophilic Leukemia (RBL-2H3) Cells
TLDR
Flotillin-1 is an essential molecule in IgE receptor-mediated mast cell activation, and regulates the kinase activity of Lyn in lipid rafts, which is involved in the process of Lyn activation.
Rat basophilic leukemia cells express syntaxin-3 and VAMP-7 in granule membranes.
TLDR
The expression and the localization of SNARE proteins including VAMP-7 which is insensitive to tetanus toxin are investigated and suggest the involvement ofSNARE proteins in the degranulation of RBL-2H3 cells.
Improved cellular uptake of chitosan-modified PLGA nanospheres by A549 cells.
TLDR
It is demonstrated that chitosan (CS)-modified PLGANSs (CS-PLGA NSs) are preferentially taken up by human lung adenocarcinoma cells (A549), suggesting a clathrin-mediated endocytic process.
Calcium Responses in Fibroblasts from Asymptomatic Members of Alzheimer's Disease Families
TLDR
It is reported here that alterations in IP3-mediated calcium responses are present in a large proportion of AD family members before clinical symptoms of Alzheimer's disease are present, which was not the case if such members later "escaped" AD symptoms.
Complexin II facilitates exocytotic release in mast cells by enhancing Ca2+ sensitivity of the fusion process
TLDR
The results suggest that complexin II regulates exocytosis positively by translocating to the plasma membrane and enhancing the Ca2+ sensitivity of fusion machinery, although this translocation to the Plasma membrane is not sufficient to trigger exocyTotic membrane fusion.
Microtubule involvement in the intracellular dynamics for gene transfection mediated by cationic liposomes
TLDR
It is demonstrated that the liposome–DNA complexes are transported to lysosomes by a microtubule-mediated pathway, and the effects of nocodazole and taxol on transfection efficiency can be explained by failure of the transport of the lipo-DNA complexes to l Lysosomes where DNAs are degraded.
Dynamics of intracellular granules with CD63-GFP in rat basophilic leukemia cells.
TLDR
Analysis of the movement of each granule provided a new insight into the elementary process of degranulation and showed that the granules are able to reach the plasma membranes in 2 or 3 min if the diameter of the cells is 20 microm.
ZIP2 Protein, a Zinc Transporter, Is Associated with Keratinocyte Differentiation
TLDR
The results of this study suggest that ZIP2, a zinc transporter expressed specifically in the epidermis, and zinc taken up by ZIP2 are necessary for the differentiation of keratinocytes.
Transfer of small interfering RNA by single-cell electroporation in cerebellar cell cultures
TLDR
The single-cell electroporation of siRNA could be a simple but effective tool for silencing gene expression in individual cells in neuronal primary cultures, but both gene-silencing and off-target effects of si RNA introduced by this method may differ between neuronal cell types, and the parameters of single- cell electropation should be optimized in each cell type.
Biosurfactant mannosyl-erythritol lipid inhibits secretion of inflammatory mediators from RBL-2H3 cells.
TLDR
MELs have anti-inflammatory action inhibiting the secretion of inflammatory mediators from mast cells and inhibited the phosphorylation of SNARE proteins that is crucial for not only exocytosis but also intracellular vesicular trafficking.
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