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FRET – Förster Resonance Energy Transfer
Quantum dots: bright and versatile in vitro and in vivo fluorescence imaging biosensors.
Different QD-based imaging applications will be discussed from the technological and the biological point of view, ranging from super-resolution microscopy and single-particle tracking over in vitro cell and tissue imaging to in vivo investigations.
Energy Transfer with Semiconductor Quantum Dot Bioconjugates: A Versatile Platform for Biosensing, Energy Harvesting, and Other Developing Applications.
This work discusses how QDs are steadily revolutionizing the development of new biosensors along with a myriad of other photonically active nanomaterial-based bioconjugates, and how this field has grown over a relatively short time span.
Rapid and Multiplexed MicroRNA Diagnostic Assay Using Quantum Dot-Based Förster Resonance Energy Transfer.
Clinical applicability is demonstrated by sensitive multiplexed quantification of three miRNAs at low (2 to 10 nM) and varying concentrations in samples that contained up to 10% serum.
Fluorescence in nanobiotechnology: sophisticated fluorophores for novel applications.
This review covers the growing utility of different classes of fluorophores in nanobiotechnology, from both a photophysical and a chemical perspective.
Time-Resolved Analysis of a Highly Sensitive Förster Resonance Energy Transfer Immunoassay Using Terbium Complexes as Donors and Quantum Dots as Acceptors
- N. Hildebrandt, L. Charbonnière, H. Löhmannsröben
- Physics, ChemistryJournal of biomedicine & biotechnology
- 10 September 2007
A detection limit of 18 attomol QDs within the immunoassay is accomplished, an improvement of more than two orders of magnitude compared to commercial systems, as well as detailed decay time analysis of the FRET process.
Self-Assembled Gold Nanoclusters for Bright Fluorescence Imaging and Enhanced Drug Delivery.
- Akram Yahia-Ammar, D. Sierra, F. Mérola, N. Hildebrandt, X. le Guével
- Biology, Materials ScienceACS nano
- 5 February 2016
A simple, fast, and robust protocol of cationic polymer-mediated gold nanocluster self-assembly into nanoparticles (NPs) of ca.
Quantum dots as simultaneous acceptors and donors in time-gated Förster resonance energy transfer relays: characterization and biosensing.
- W. R. Algar, D. Wegner, Igor L. Medintz
- Biology, ChemistryJournal of the American Chemical Society
- 5 January 2012
The utility of incorporating QDs into this type of time-gated energy transfer configuration was demonstrated in prototypical bioassays for monitoring protease activity and nucleic acid hybridization; the latter included a dual target format where each orthogonal FRET step transduced a separate binding event.
Luminescent terbium complexes: Superior Förster resonance energy transfer donors for flexible and sensitive multiplexed biosensing
Upconverting nanoparticle to quantum dot FRET for homogeneous double-nano biosensors
It is demonstrated that UCNP-to-QD FRET systems can be used for rapid homogeneous bioassays, which are essential tools for clinical diagnostics and offer a large potential for spectroscopy and imaging-based biosensing beyond in vitro diagnostics.