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The end of an old hypothesis: the pseudomonas signaling molecules 4-hydroxy-2-alkylquinolines derive from fatty acids, not 3-ketofatty acids.
It is demonstrated that octanoic acid is directly incorporated into HHQ, the direct precursor of PQS, and uncovers promising targets for the development of specific antivirulence drugs to combat this opportunistic pathogen.
Semi-rational approaches to engineering enzyme activity: combining the benefits of directed evolution and rational design.
Efficient sampling of mutations likely to affect enzyme function has been conducted both experimentally and computationally, with remarkable improvements in substrate selectivity and specificity and in the de novo design of enzyme activities within scaffolds of known structure.
NMR investigation of Tyr105 mutants in TEM-1 beta-lactamase: dynamics are correlated with function.
The results support previous observations suggesting that internal motions play a role in promoting protein function and suggest that short and long range residue motions could play an important catalytic role in TEM-1 beta-lactamase.
Design and characterization of novel cell-penetrating peptides from pituitary adenylate cyclase-activating polypeptide.
This study investigated the use of novel cell-penetrating peptides (CPPs) derived from the endogenous neuropeptide Pituitary adenylate cyclase-activating polypeptides (PACAP) and contributed to the identification of new members of the CPP family.
Cytosolic galectin-7 impairs p53 functions and induces chemoresistance in breast cancer cells
This study suggests that targeting CRD-independent cytosolic gal-7 in breast cancer cells may be a valuable strategy for the treatment of this disease and will complement efforts towards improving selectivity of targeted anticancer agents.
The flexibility of a distant loop modulates active site motion and product release in ribonuclease A.
Together, these NMR and biochemical experiments provide additional insight into the mechanism of millisecond motions in the RNase A catalytic cycle.
Combining chitinase C and N-acetylhexosaminidase from Streptomyces coelicolor A3(2) provides an efficient way to synthesize N-acetylglucosamine from crystalline chitin.
This chitin hydrolysis yield represents one of the most efficient enzyme bioconversion of chitopolysaccharides to GlcNAc characterized to date, making the S. coelicolor ScChiC-ScHEX pair a potentially suitable contender for the viable industrial production of this important bioactive compound.
Short-Chain Flavor Ester Synthesis in Organic Media by an E. coli Whole-Cell Biocatalyst Expressing a Newly Characterized Heterologous Lipase
The whole-cell biocatalyst showed excellent tolerance to alcohol and short-chain fatty acid denaturation, and the recombinant lipase showed good affinity toward glyceryl trioctanoate, which shows promising potential for an industrial approach aimed at the biosynthesis of short- chain esters.
Effect of combination of essential oils and bacteriocins on the efficacy of gamma radiation against Salmonella Typhimurium and Listeria monocytogenes
The combined EO with nisin and irradiation could increase the RS of L. monocytogenes and Salmonella Typhimurium.
Combinatorial active‐site variants confer sustained clavulanate resistance in BlaC β‐lactamase from Mycobacterium tuberculosis
Bacterial resistance to β‐lactam antibiotics is a global issue threatening the success of infectious disease treatments worldwide. Mycobacterium tuberculosis has been particularly resilient to