An efficient recombination system for chromosome engineering in Escherichia coli.
- D. Yu, H. Ellis, E. C. Lee, N. Jenkins, N. Copeland, D. Court
- Biology, EngineeringProceedings of the National Academy of Sciences…
- 23 May 2000
A recombination system has been developed for efficient chromosome engineering in Escherichia coli by using electroporated linear DNA using a defective lambda prophage, which will be especially useful for the engineering of large bacterial plasmids such as those from bacterial artificial chromosome libraries.
Simple and highly efficient BAC recombineering using galK selection
- S. Warming, N. Costantino, D. Court, N. Jenkins, N. Copeland
- BiologyNucleic Acids Research
- 24 February 2005
Three new recombineering strains are described that allow bacterial artificial chromosomes (BACs) to be modified using galK positive/negative selection, and it is shown how galK selection can be used to rapidly introduce point mutations, deletions and loxP sites into BAC DNA and thus facilitate functional studies of SNP and/or disease-causing point mutations.
Arc, a growth factor and activity-regulated gene, encodes a novel cytoskeleton-associated protein that is enriched in neuronal dendrites
A highly efficient Escherichia coli-based chromosome engineering system adapted for recombinogenic targeting and subcloning of BAC DNA.
The ability to modify or subclone large fragments of genomic DNA with precision should facilitate many kinds of genomic experiments that were difficult or impossible to perform previously and aid in studies of gene function in the postgenomic era.
Onset and Progression in Inherited ALS Determined by Motor Neurons and Microglia
Onset and progression of amyotrophic lateral sclerosis represent distinct disease phases defined by mutant action within different cell types to generate non–cell-autonomous killing of motor neurons; these findings validate therapies, including cell replacement, targeted to the non-neuronal cells.
A highly efficient recombineering-based method for generating conditional knockout mutations.
This method is fast, efficient, and reliable and makes it possible to generate cko-targeting vectors in less than 2 wk and should also facilitate the generation of knock-in mutations and transgene constructs, as well as expedite the analysis of regulatory elements and functional domains in or near genes.
Lymphoproliferation disorder in mice explained by defects in Fas antigen that mediates apoptosis
- R. Watanabe‐Fukunaga, C. Brannan, N. Copeland, N. Jenkins, S. Nagata
- Biology, MedicineNature
- 26 March 1992
The Ipr mice develop lymphadenopathy and suffer from a systemic lupus erythematosus-like autoimmune disease, indicating an important role for Fas antigen in the negative selection of autoreactive T cells in the thymus.
Six3, a murine homologue of the sine oculis gene, demarcates the most anterior border of the developing neural plate and is expressed during eye development.
The isolation of a sequence-related gene referred to as Six3 is reported, which is one of the most anterior homeobox gene reported to date and supports the idea that mammals and insects share control genes such as eyeless/Pax6 and also possibly other members of the regulatory cascade required for eye morphogenesis.
Identification of Vangl2 and Scrb1 as planar polarity genes in mammals
A role for the PCP pathway in planar polarization in mammals is demonstrated, and a mutation in Vangl2, a mammalian homologue of the Drosophila PCP gene, is shown to results in significant disruptions in the polarization of stereociliary bundles in mouse cochlea.