Technical advance: An estrogen receptor-based transactivator XVE mediates highly inducible gene expression in transgenic plants.
Neither toxic nor adverse physiological effects of the XVE system have been observed in transgenic Arabidopsis plants under all the conditions tested and it appears to be a reliable and efficient chemical-inducible system for regulating transgene expression in plants.
A postgermination developmental arrest checkpoint is mediated by abscisic acid and requires the ABI5 transcription factor in Arabidopsis
- L. Lopez-Molina, S. Mongrand, N. Chua
- Environmental ScienceProceedings of the National Academy of Sciences…
- 3 April 2001
It is reported that the basic leucine zipper transcription factor ABI5 confers an enhanced response to exogenous ABA during germination, and seedling establishment, as well as subsequent vegetative growth.
Cucumber mosaic virus-encoded 2b suppressor inhibits Arabidopsis Argonaute1 cleavage activity to counter plant defense.
- Xiuren Zhang, Y. Yuan, N. Chua
- BiologyGenes & Development
- 1 December 2006
It is concluded that 2b blocks AGO1 cleavage activity to inhibit miRNA pathways, attenuate RNA silencing, and counter host defense, providing insight on the molecular arms race between host antiviral RNAsilencing and virus counterdefense.
ABI5 acts downstream of ABI3 to execute an ABA-dependent growth arrest during germination.
- L. Lopez-Molina, S. Mongrand, D. McLachlin, B. Chait, N. Chua
- Environmental ScienceThe Plant Journal
- 1 November 2002
Results indicate that ABI5 acts downstream of ABI3 to reactivate late embryogenesis programmes and to arrest growth of germinating embryos.
Arabidopsis NAC1 transduces auxin signal downstream of TIR1 to promote lateral root development.
NAC1, a new member of the NAC family, is induced by auxin and mediates auxin signaling to promote lateral root development and can restore lateral root formation in the auxin-response mutant tir1, indicating that NAC1 acts downstream of TIR1.
Actin Depolymerizing Factor (ADF/Cofilin) Enhances the Rate of Filament Turnover: Implication in Actin-based Motility
- M. Carlier, V. Laurent, D. Pantaloni
- BiologyJournal of Cell Biology
- 24 March 1997
The function of ADF is not to sequester G-actin, but uses ATP hydrolysis in actin assembly to enhance filament dynamics and increase in the rate of actin-based motile processes.
A glucocorticoid-mediated transcriptional induction system in transgenic plants.
The results indicate that a stringent chemical control of transcription can be achieved in plants with the GVG system, and can also be regulated by DEX in transgenic Arabidopsis plants.
A GFP-mouse talin fusion protein labels plant actin filaments in vivo and visualizes the actin cytoskeleton in growing pollen tubes.
- B. Kost, P. Spielhofer, N. Chua
- BiologyThe Plant Journal
- 1 November 1998
It is found that transient and stable expression of the talin actin-binding domain fused to the C-terminus of the green fluorescent protein (GFP-mTn) can visualize the actin cytoskeleton in different types of living plant cells without affecting cell morphology or function.
PSEUDO-RESPONSE REGULATORS 9, 7, and 5 Are Transcriptional Repressors in the Arabidopsis Circadian Clock[W][OA]
- N. Nakamichi, T. Kiba, Rossana Henriques, T. Mizuno, N. Chua, H. Sakakibara
- Biology, Environmental ScienceThe Plant Cell
- 1 March 2010
The results suggest that the repressor activities of PRR9, PRR7, and PRR5 on the CCA1 and LHY promoter regions constitute the molecular mechanism that accounts for the role of these proteins in the feedback loop of the circadian clock.
Rac Homologues and Compartmentalized Phosphatidylinositol 4, 5-Bisphosphate Act in a Common Pathway to Regulate Polar Pollen Tube Growth
- B. Kost, E. Lemichez, N. Chua
- BiologyJournal of Cell Biology
- 19 April 1999
Results indicate that Rac and PtdIns 4, 5-P2 act in a common pathway to control polar pollen tube growth and provide direct evidence for a function of Ptdins 4,5-P1 compartmentalization in the regulation of this process.
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