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Cellular effects of leishmanial tubulin inhibitors on L. donovani.
TLDR
Tubulin is validated as a suitable antileishmanial drug target and the cellular effects of the mammalian antimicrotubule agents ansamitocin P3, taxol, and hemiasterlin on Leishmania donovani promastigotes provide criteria to assess the cellular mechanism of action of new candidate antileishesmanial agents.
Effect of the concentration of Ca2+ in the suspending medium on the responses of human and rabbit platelets to aggregating agents.
The effect of the concentration of Ca2+ in the suspending medium of human and rabbit platelets on aggregation, release of 14C-serotonin, and TXB2 formation in response to ADP, thrombin,
Agglutination of rabbit platelets in plasma by the thrombin inhibitor D-phenylalanyl-L-prolyl-L-arginyl chloromethyl ketone.
TLDR
FPRCH2Cl cannot be used as an anticoagulant for rabbit blood when platelets are to be examined, and has a profound effect on the platelet count in vivo in rabbits.
Palmitic acid-labeled lipids selectively incorporated into platelet cytoskeleton during aggregation
TLDR
Investigation of the nature of the labeled lipids by thin-layer and column chromatography found no evidence for the incorporation of diacylglycerol and palmitic acid into the cytoskeleton, contrary to a report in the literature.
Incorporation of lipids labeled with various fatty acids into the cytoskeleton of aggregating platelets
TLDR
It is suggested that ceramide and phospholipids that are readily labeled with saturated fatty acids are selectively incorporated into the cytoskeleton during the early stages of aggregation and may be specifically associated with the points of contact between platelets.
Endogenous Gene Tagging of pfr2 and pfr5 in Trypanosoma cruzi Using CRISPR/Cas9
TLDR
A CRISPR/Cas9 endogenous gene tagging protocol is adapted to tag pfr5 and investigate the subcellular localization of the protein, which constitutes a unique set of proteins that are known to be immunogenic and provide protective immunity to T. cruzi.