Share This Author
Development and characterization of a rainbow trout liver cell line expressing cytochrome P450-dependent monooxygenase activity
Activity for the enzyme, 7-ethoxyresorufin O-deethylase (EROD), increased dramatically in RTL-W1 upon their exposure to increasing concentrations of either β-naphthoflavone (BNF) or 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD).
Ecotoxicology and innate immunity in fish.
Development of a cell line from primary cultures of rainbow trout, Oncorhynchus mykiss (Walbaum), gills
Collagenase was used to prepare primary cell cultures from rainbow trout gills, which led to the development of a cell line, RTgill-W1, which was found to be predominantly polygonal or epithelial-like at confluency and contaminated with mycoplasma.
Identification and Bioactivities of IFN-γ in Rainbow Trout Oncorhynchus mykiss: The First Th1-Type Cytokine Characterized Functionally in Fish1
The present study has demonstrated for the first time a functional IFN-γ homologue in a fish species, strongly suggesting a conserved Th1 immune response is most likely present in lower vertebrates.
Methodology for demonstrating and measuring the photocytotoxicity of fluoranthene to fish cells in culture.
Identification and analysis of an interleukin 8-like molecule in rainbow trout Oncorhynchus mykiss.
Ability of polycyclic aromatic hydrocarbons to induce 7-ethoxyresorufin-o-deethylase activity in a trout liver cell line.
- N. Bols, K. Schirmer, E. M. Joyce, D. Dixon, B. Greenberg, J. Whyte
- ChemistryEcotoxicology and environmental safety
- 1 September 1999
Along with 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), 24 unsubstituted polycyclic aromatic hydrocarbons (PAHs) were evaluated for their ability to induce 7-ethoxyresorufin-o-deethylase (EROD)…
Chapter 2 Use of fish cell lines in the toxicology and ecotoxicology of fish. Piscine cell lines in environmental toxicology
The use of Fish Cells in Ecotoxicology: The Report and Recommendations of ECVAM Workshop 471, 2
- A. Castaño, N. Bols, H. Segner
- Environmental ScienceAlternatives to laboratory animals : ATLA
- 1 May 2003
This is the report of the forty-seventh of a series of workshops organised by the European Centre for the Validation of Alternative Methods (ECVAM), held in Angera, Italy, on 22–24 October 2001, under the co-chairmanship of Argelia Castaño and Helmut Segner.
Synthesis of polybrominated diphenyl ethers and their capacity to induce CYP1A by the Ah receptor mediated pathway.
- G. Chen, A. Konstantinov, B. Chittim, E. M. Joyce, N. Bols, N. Bunce
- Chemistry, BiologyEnvironmental science & technology
- 14 August 2001
This work synthesized a number of PBDE congeners, studied their affinity for rat hepatic Ah receptor through competitive binding assays, and determined their ability to induce hepatic cytochrome P-450 enzymes by means of EROD assays in human, rat, chick, and rainbow trout cells.