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Development and characterization of a rainbow trout liver cell line expressing cytochrome P450-dependent monooxygenase activity
Activity for the enzyme, 7-ethoxyresorufin O-deethylase (EROD), increased dramatically in RTL-W1 upon their exposure to increasing concentrations of either β-naphthoflavone (BNF) or 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Expand
Ecotoxicology and innate immunity in fish.
Advances in basic knowledge of fish innate immunity should lead to improvements in monitoring fish health and predicting the impact of toxicants on fish populations, which is a fundamental ecotoxicological goal. Expand
Identification and Bioactivities of IFN-γ in Rainbow Trout Oncorhynchus mykiss: The First Th1-Type Cytokine Characterized Functionally in Fish1
The present study has demonstrated for the first time a functional IFN-γ homologue in a fish species, strongly suggesting a conserved Th1 immune response is most likely present in lower vertebrates. Expand
Methodology for demonstrating and measuring the photocytotoxicity of fluoranthene to fish cells in culture.
The indicator dyes alamar Blue and 5-carboxyfluorescein diacetate acetoxymethyl ester were used to quantify cytotoxicity in two different ways-singly in two separate assays, and mixed together in a novel single assay, which saved time and material. Expand
Chapter 2 Use of fish cell lines in the toxicology and ecotoxicology of fish. Piscine cell lines in environmental toxicology
This chapter reviews the uses of one type, cell lines, in fish environmental toxicology and describes the nature of animal cell cultures, and explains the advantages ofAnimal cell cultures and toxicology applications and ecotoxicology applications of fish cell lines. Expand
Identification and analysis of an interleukin 8-like molecule in rainbow trout Oncorhynchus mykiss.
An interleukin 8 (IL-8) homologue has been identified in the rainbow trout Oncorhynchus mykiss and RT-PCR reveals a low level constitutive expression of the IL-8 homologue in many tissues, including spleen, heart, liver, head kidney and gill. Expand
The use of Fish Cells in Ecotoxicology: The Report and Recommendations of ECVAM Workshop 471, 2
This is the report of the forty-seventh of a series of workshops organised by the European Centre for the Validation of Alternative Methods (ECVAM), held in Angera, Italy, on 22–24 October 2001, under the co-chairmanship of Argelia Castaño and Helmut Segner. Expand
Development of a cell line from primary cultures of rainbow trout, Oncorhynchus mykiss (Walbaum), gills
Collagenase was used to prepare primary cell cultures from rainbow trout gills, which led to the development of a cell line, RTgill-W1, which was found to be predominantly polygonal or epithelial-like at confluency and contaminated with mycoplasma. Expand
Ability of polycyclic aromatic hydrocarbons to induce 7-ethoxyresorufin-o-deethylase activity in a trout liver cell line.
Along with 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), 24 unsubstituted polycyclic aromatic hydrocarbons (PAHs) were evaluated for their ability to induce 7-ethoxyresorufin-o-deethylase (EROD)Expand
Synthesis of polybrominated diphenyl ethers and their capacity to induce CYP1A by the Ah receptor mediated pathway.
This work synthesized a number of PBDE congeners, studied their affinity for rat hepatic Ah receptor through competitive binding assays, and determined their ability to induce hepatic cytochrome P-450 enzymes by means of EROD assays in human, rat, chick, and rainbow trout cells. Expand