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SPIDER image processing for single-particle reconstruction of biological macromolecules from electron micrographs
TLDR
This protocol describes the reconstruction of biological molecules from the electron micrographs of single particles using the image-processing software SPIDER, and a method to look for homogeneous subsets when multiple known conformations of the molecule may coexist. Expand
Mutations in myosin heavy chain 11 cause a syndrome associating thoracic aortic aneurysm/aortic dissection and patent ductus arteriosus
TLDR
Human MYH11 gene mutations provide the first example of a direct change in a specific SMC protein leading to an inherited arterial disease. Expand
The effect of overabundant projection directions on 3D reconstruction algorithms.
TLDR
A strategy for quantitative comparison and optimization of 3D reconstruction algorithms is developed and it is shown that the elongation artifacts that had been previously reported can be strongly reduced. Expand
Structural basis for the PufX-mediated dimerization of bacterial photosynthetic core complexes.
TLDR
3D reconstruction at 12 A by cryoelectron microscopy of the core complex of Rhodobacter (Rba.) sphaeroides revealing that PufX in Rba. Expand
Structure of the filamentous phage pIV multimer by cryo-electron microscopy.
TLDR
It is proposed that the pIV multimer undergoes a large conformational change during phage transport, with reorganization of the central domain to open the pore, and widening at the N-ring in order to accommodate the 6.5 nm diameter phage particle. Expand
Intramolecular localization of the functional units of Sepia officinalis hemocyanin by immunoelectron microscopy.
TLDR
An analysis of the quaternary structure considering the possible locations of the 80 FUs and postulating a single type of subunit shows that 80 possibilities of paths still exist for the polypeptide chain. Expand
Structure of the Acidianus Filamentous Virus 3 and Comparative Genomics of Related Archaeal Lipothrixviruses
TLDR
Four novel filamentous viruses with double-stranded DNA genomes, namely, Acidianus filamentous virus 3 (AFV3), AFV6, AFV7, and AFV8, have been characterized and reveal a high level of conservation in both gene content and gene order over large regions, with this similarity extending partly to the earlier described betalipothrixvirus Sulfolobus islandicus filamentousirus. Expand
Preservation of protein globules and peptidoglycan in the mineralized cell wall of nitrate-reducing, iron(II)-oxidizing bacteria: a cryo-electron microscopy study.
TLDR
It is shown that the use of cryo-TEM instead of conventional microscopy provides detailed information of the successive iron biomineralization stages in anaerobic nitrate-reducing iron-oxidizing bacteria and offers a detailed view of the exceptional preservation of protein globules and the peptidoglycan within the Fe-mineralized cell walls of these bacteria. Expand
Divalent ion-dependent swelling of tomato bushy stunt virus: a multi-approach study.
TLDR
Cryo-electron microscopy provides an invaluable starting point for the analysis of the time-resolved swelling process using the scattering data and a procedure has been developed to compute the SAXS pattern from the 3D reconstruction for comparison with experimental data. Expand
Quaternary structure of Octopus vulgaris hemocyanin. Three-dimensional reconstruction from frozen-hydrated specimens and intramolecular location of functional units Ove and Ovb.
TLDR
Results suggest that the direction of the polypeptide chains in the cylinder wall may be only partially antiparallel, and a model of the quaternary structure is proposed with the following features. Expand
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