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Functional analysis of cis-regulatory elements within the promoter of the tobacco late pollen gene g10
TLDR
The function of a conserved GTGA motif shared between the tobacco g10 and tomato lat56 promoters was demonstrated in g10, providing further functional evidence for the conservation of mechanisms for the regulation of late pollen genes across species. Expand
Functional architecture of a late pollen promoter: pollen-specific transcription is developmentally regulated by multiple stage-specific and co-dependent activator elements
TLDR
Results demonstrate that transcriptional activation of lat52 is controlled by a complex of pollen-specific cis-regulatory elements which cooperate to achieve maximum levels of gene expression throughout pollen maturation. Expand
The structure of an integrin/talin complex reveals the basis of inside‐out signal transduction
TLDR
This work reports the first structure of talin bound to an authentic full‐length β integrin tail and identifies a positively charged surface on the talin F2 domain that precisely orients talin to disrupt the heterodimeric integrin transmembrane (TM) complex. Expand
The Structure of the Talin Head Reveals a Novel Extended Conformation of the FERM Domain
TLDR
It is suggested that the extended conformation of the talin head allows simultaneous binding to integrins via F3 and to PtdIns(4,5)P2-enriched microdomains via basic residues distributed along one surface of the Talin head, and that these multiple interactions are required to stabilize Integrin in the activated state. Expand
RIAM and Vinculin Binding to Talin Are Mutually Exclusive and Regulate Adhesion Assembly and Turnover*
TLDR
The structural characterization of the talin rod is reported, which proposes a model in which RIAM binding to R2R3 initially recruits talin to membranes where it activates integrins and recruits vinculin to stabilize the complex. Expand
Talin determines the nanoscale architecture of focal adhesions
TLDR
It is demonstrated that talin plays a key structural role in regulating the nanoscale architecture of FAs, akin to a molecular ruler, in a remarkably modular manner. Expand
The structure of the C-terminal actin-binding domain of talin
TLDR
Electron microscopy provides direct evidence for binding of the dimer to F‐actin and indicates that it binds to three monomers along the long‐pitch helix of the actin filament. Expand
Mechanotransduction In Vivo by Repeated Talin Stretch-Relaxation Events Depends upon Vinculin
The focal adhesion protein talin undergoes cycles of stretching and relaxation in living cells, suggesting a role in the transduction of mechanical into biochemical signals.
Structure of a double ubiquitin-like domain in the talin head: a role in integrin activation
TLDR
Solution structures of F0, F1 and of the F0F1 double domain are reported, suggesting that extensive interactions between the talin FERM domain and acidic membrane phospholipids are required to orientate the F ERM domain such that it can activate integrins. Expand
Activation of a vinculin‐binding site in the talin rod involves rearrangement of a five‐helix bundle
TLDR
Activation of the VBS in talin and the recruitment of vinculin may support the maturation of small integrin/talin complexes into more stable adhesions. Expand
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