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Collembola as alternative prey sustaining spiders in arable ecosystems: prey detection within predators using molecular markers
It is reported the first use of DNA‐based techniques to detect predation by arthropods on natural populations of prey in the field and it was possible to demonstrate that the spiders were exercising prey choice. Expand
Universal and blocking primer mismatches limit the use of high‐throughput DNA sequencing for the quantitative metabarcoding of arthropods
The results show that reads of all species were recovered after PCR enrichment at the authors' control conditions and high‐throughput sequencing, and show that the four factors considered biased the final proportions of the species to some degree. Expand
Development of sequence amplified characterized region (SCAR) markers of Helicoverpa armigera: a new polymerase chain reaction‐based technique for predator gut analysis
A method is described for the development of DNA markers for detection of Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) in predator gut analysis, based on sequence characterized amplifiedExpand
Detecting Cacopsylla pyricola (Hemiptera: Psyllidae) in predator guts using COI mitochondrial markers.
Two molecular markers developed from C. pyricola cytochrome oxidase I fragments failed to amplify DNA from representative species of the Coccinellidae, Chrysopidae, Hemerobiidae, Anthocoridae, Miridae, Salticidae, Aphididae, Tetranychidae and the Tortricidae, suggesting their suitability for general trophic studies. Expand
Lygus hesperus and L. lineolaris (Hemiptera: Miridae), phytophages, zoophages, or omnivores: evidence of feeding adaptations suggested by the salivary and midgut digestive enzymes.
This work studied the digestive enzymes in the salivary gland complex and guts of L. hesperus and L. lineolaris to elucidate the feeding mechanism and to determine the relative zoophagous and phytophragous potential of each species. Expand
Detection, identification and geographical distribution of European corn borer larval parasitoids using molecular markers
The molecular evaluation of parasitism was compared with the traditional method of maintaining O. nubilalis populations in controlled conditions before breaking off the diapause, suggesting an underestimation by the traditional rearing protocol. Expand
Development of specific ITS markers for plant DNA identification within herbivorous insects
A PCR approach based on the development of specific primers was developed as a self-marking technique to detect plant DNA within the gut of one heteropteran omnivorous predator and two lepidopteran pest species and showed a higher detection of tomato DNA in females and nymphs than males. Expand
Developing SCAR markers to study predation on Trialeurodes vaporariorum
DNA markers of Trialeurodes vaporariorum were developed to detect remains of these whitefly in the gut of the predator Dicyphus tamaninii, but detection of T. vaporarIORum DNA in the predator gut was only possible using the primers that amplified the shortest fragment. Expand
Multiplex reactions for the molecular detection of predation on pest and nonpest invertebrates in agroecosystems
Species‐ and group‐specific PCR primers were developed to study predation on pest and nonpest invertebrate species by generalist carabid predators in agroecosystems and were designed to be less than 300 bp to ensure the amplification of degraded DNA in predator gut samples. Expand
Group‐specific primers for DNA‐based detection of springtails (Hexapoda: Collembola) within predator gut contents
Group‐specific, degenerate polymerase chain reaction primers for DNA‐based detection of springtails within predator gut contents have been developed for the first time and could facilitate studies of trophic links between springtails and generalist arthropod predators worldwide. Expand