N. V. Yartseva

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194 This is the first study to perform a direct transfer of the pGA482:ipt plasmid (14.6 kb) bearing the cytokii nin biosynthesis gene into the cells of phototrophic purple bacteria Rhodopseudomonas palustris and Rhodobacter sphaeroides by electroporation. PCR analysis showed the presence of the ipt gene in the template DNA isolated from the transformed(More)
The unicellular green alga Chamydomonas reinhardtii, as distinct from higher plants synthesizing chlorophyll (Chl) only in the light, produces it also in darkness. The mechanisms of dark Chl biosynthesis were studied by biochemical and genetic methods on C. reinhardtii pigment LTS3 mutants, their revertants, and reverse mutants; LTS3 gene encodes a(More)
Phototrophic non-sulfur bacteria (PNSB) are close relatives of cytokinin producing agrobacteria. However, the sequenced PNSB genomes do not have the ipt gene sequences involved in the cytokinin biosynthesis. In Rhodobacter sphaeroides and Rhodopseudomonas palustris transformed with ipt, we previously observed the substantial morphological and physiological(More)
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