N. V. Epifanova

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Rotaviruses were detected in 58 among 194 children with nonbacterial gastroenteritis whose feces were examined by direct electron microscopy and immune electron microscopy (IEM). Identification of Coxsackie B viruses isolated from patients with infectious-allergic myocarditis by IEM and the neutralization tests gave similar results (Coxsackie B6). Besides,(More)
1370 natural strains of human rotaviruses which circulated on the territory of Nizhny Novgorod during the period of 1984-1996 were differentiated with the use of electrophoretic typing of RNA. 58 electrophoretic types of RNA were detected; of these, 8 types dominated in the populations. The specific features of the circulation of strains with different(More)
The findings of epidemiological study of rotavirus infections among children in Gorki over the period of 1981-1985 are analyzed. The age of the patients is specified, as are the disease seasonal patterns and the incidence rates of its manifest and asymptomatic forms, and of carriership cases among healthy children in different foci of this infection.
A procedure based on polymerase chain reaction was developed for the study of rotaviruses. A full-length cDNA copy of the gene coding for major neutralizing glycoprotein VP7 of a human rotavirus isolate 1407 with the "long" electrophoretype (classified as the first electrophoretype) was cloned and sequenced. The primary structure of glycoprotein VP7 of(More)
RT-PCR was used to determine G (VP7) and P (VP4) genotypes of group A rotavirus found in 2454 children with diarrhea in 1997-2005. Eight G[P] combinations, including G1-4 serotypes and P[4, 6, 8, 9] genotypes, were identified. The P[8] genotype was presented by the subtypes P[8]-1 and P[8]-2. In 1997-2005, the spectrum and distribution of G[P] types were as(More)
A collection of rotavirus samples collected over a 26-year period was examined to study the dynamics of change in RV strains of genotype P[8] in a geographically defined population (Nizhny Novgorod, Russia; children under 6 years) with no vaccine pressure. Phylogenetic analysis of gene VP4 (subunit VP8*) showed the presence of two lines of genotype P[8]:(More)
Double-segment profiles typical of picobirnavirus (PBV) were detected (during 1994-2001) in nucleic acids extracted from feces of children (3 cases) and calf (1 case) with diarrhea by using the method of electrophoresis. The human genomic PBV segments migrated in the polyacrylamide gel (PAAG) as dsRNA segments sized 1.7 and 2.4 kbp for small and large(More)
The electrophoretic typing of 1,370 rotavirus strains revealed the presence of group C rotaviruses in 1.3% of cases. In the Arkhangelsk region group C rotaviruses were detected in 34.4% of cases. The detected group C rotaviruses were shown to be no different in their morphology from typical ones; they were characterized by the variability of their genome(More)
Comparative studies of the yield of stable and unstable chromosome aberrations have been carried out in human lymphocytes gamma irradiated in vitro within the doze range 0.1--1.0 Gy. It was observed that the yield of unstable chromosome aberrations (dicentrics and centric rings) is satisfactorily described for discovered dose range with linear-quadratic(More)
A method is suggested for the detection of the human rotavirus VP4 gene by RT-PCR. The method is universal for all [P] types of group A rotavirus in clinical samples. As against RNA electrophoresis and ELISA, it provides for a more accurate detection of rotaviruses by 18.8 and 26.5%, respectively. It was established by RT-PCR in February, 2002, in Nizhny(More)