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The expression of oncofetal H19 RNA and its localization/cellular source was analyzed in synovial tissue (ST) and isolated synovial macrophages (Mphi) or synovial fibroblasts (SFBs) by reverse transcriptase-polymerase chain reaction (RT-PCR), in situ hybridization, and immunohistochemistry. RT-PCR showed significantly higher H19 expression in ST from… (More)
Nuclease S1 mapping analyses were performed in order to detect processing intermediates of pre-23S rRNA from Thermus thermophilus HB8. Two processing sites were identified downstream the start of transcription and several consecutive cleavage sites are associated with the mature 5'-end. In the 3'-flanking region one "primary" site and two cleavages which… (More)
A dnaZX-Wke open reading frame downstream from the Bacillus subtilis scRNA gene In a B. subtilis 1.5 kb EcoR I DNA-fragment containing the 3'-moiety of the scRNA gene (underlined) (1) an ORF"honblogous to dnaSC from E. coli (2) was detected, suggesting the ORF to represent the gene for ENA polymerase HI. Potential regulatory elements are indicated.