N. Tyagi

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Deoxyhypusine hydroxylase (DOHH) catalyzes the final step in the post-translational synthesis of an unusual amino acid hypusine (N(€)-(4-amino-2-hydroxybutyl) lysine), which is present on only one cellular protein, eukaryotic initiation factor 5A (eIF5A). We present here the molecular and structural basis of the function of DOHH from the protozoan parasite,(More)
While phosphotyrosine modification is an established regulatory mechanism in eukaryotes, it is less well characterized in bacteria due to low prevalence. To gain insight into the extent and biological importance of tyrosine phosphorylation in Escherichia coli, we used immunoaffinity-based phosphotyrosine peptide enrichment combined with high resolution mass(More)
Interaction of non-structural protein 5A (NS5A) of Hepatitis C virus (HCV) with human kinases namely, casein kinase 1α (ck1α) and protein kinase R (PKR) have different functional implications such as regulation of viral replication and evasion of interferon induced immune response respectively. Understanding the structural and molecular basis of(More)
BACKGROUND Overwhelming majority of the Serine/Threonine protein kinases identified by gleaning archaeal and eubacterial genomes could not be classified into any of the well known Hanks and Hunter subfamilies of protein kinases. This is owing to the development of Hanks and Hunter classification scheme based on eukaryotic protein kinases which are highly(More)
Finding meaningful information among the billions of information resources on the Web is a difficult task due to growing popularity of the Internet. The future of World Wide Web (WWW) is the Semantic Web, where ontologies are used to assign (agreed) meaning to the content of the Web. On the Semantic Web, data will inevitably be linked to many different(More)
A focused crawler downloads web pages that are relevant to a user specified topic. The downloaded documents are indexed with a view to optimize speed and performance in finding relevant documents for a search query at the search engine side. However, the information will be more relevant if the context of the topic is also made available to the retrieval(More)
Takifugu rubripes is teleost fish widely used in comparative genomics to understand the human system better due to its similarities both in number of genes and structure of genes. In this work we survey the fugu genome, and, using sensitive computational approaches, we identify the repertoire of putative protein kinases and classify them into groups and(More)
The information on the WWW is available in various formats. The RDF and XML representation provides semantic knowledge about the document where as HTML markup only indicates the structure and layout of documents, but not the document semantics. The representation of the HTML document to semantic form can facilitate the extraction of knowledge from these(More)