N. S. Polimbetova

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The sequences of different plant viral leaders with known translation enhancer ability show partial complementarity to the central region of 18S rRNA. Such complementarity might serve as a means to attract 40S ribosomal subunits and explain in part the translation-enhancing property of these sequences. To verify this notion, we designed beta-glucuronidase(More)
S OF PRESENTATIONS ON PLANT PROTECTION ISSUES AT THE XTH INTERNATIONAL CONGRESS OF VIROLOGY August 11-16, 1996 Binyanei haOoma, Jerusalem, Israel Part2* Plenary Lectures X" ANTIVIRAL AGENTS FROM PLANT SOURCES (PLANT EXTRACTS; PROTEINS) (continued) Eff i cacy o f T r a d i t i o n a l H e r b a l Medic ines o n H e r p e s V i r u s In fec t ion in vitro a n(More)
A putative implication 3′-terminal 18S rRNA segment in the cap-independent initiation of translation on plant ribosomes was studied. It was shown that 3′-terminal segment (nucleotides 1777–1811) of 18S rRNA including the last hairpin 45 was accessible for complementary interactions within 40S ribosomal subunits. Oligonucleotides complementary to this(More)
The properties of the informosomes released from isolated nuclei of wheat embryos were studied in an in vitro system. The release of the informosomes from isolated nuclei of wheat embryos labelled with [3H]uridine is stimulated by exogeneous ATP. Optimal conditions for informosomes release from nuclei were determined. The particles obtained have(More)
Changes in the small cytoplasmic RNA (scRNA) composition have been examined in wheat embryos exposed to heat shock conditions. A novel scRNA of about 135 nucleotides in length termed as 5.3S RNA, has been detected for the first time. With a rise in temperature from 26 degrees C up to 37 degrees C, the amount of 5.3S RNA increases 5 to 6 times. It was found(More)
The binding of the 18S rRNA of the 40S subunits of wheat germ ribosomes to an oligodeoxyribonucleotide complementary to the 1112–1123 region of the central domain of this RNA molecule has been studied. The selective binding of this oligomer to the complementary RNA fragment and the inhibition of the translation of uncapped chimeric RNA containing enhancer(More)
Possible involvement of 18S rRNA fragment 1638-1650 including basements of the helices h44 and h28 and nucleotides of the ribosomal decoding site in the cap-independent translation initiation on plant ribosomes is studied. This rRNA fragment is shown to be accessible for complementary interactions within the 40S ribosomal subunit. It is found that the(More)
The possible involvement of 18S rRNA fragment 1638–1650, including basements of the helices h44 and h28, as well as nucleotides of the ribosomal decoding site in the cap-independent mode of the initiation of the translation of plant ribosomes is studied. This rRNA fragment is shown to be accessible for complementary interactions in the 40S ribosomal(More)
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