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Journals and Conferences
In vitro perfused kidneys of ovalbumin-sensitized guinea-pigs consistently released relatively large quantities of histamine when challenged with the specific antigen (mean±SEM in twelve experiments was 37.7±6.0% of total kidney histamine, maximum 70.6%, compared with a basal release of 0.5±0.46% over a comparable period) but not with non-cross-reacting… (More)
The response of cardiac mast cells in ezymically dispersed guinea-pig atrial tissue to various immune and non-immune stimuli was studied. These cells were also partially purified by density-gradient centrifugation. The release of histamine, and in some experiments leukotriene C4, was measured. The cells responsed strongly and consistently to the calcium… (More)
In guinea pig (GP) and human heart and kidney cell or tissue preparations fixed with Carnoy's fluid (but not with formol-saline), mast cells (MC) were extremely well preserved and could be detected after staining with alcain blue (AB). Although histamine content and distribution in GP and human heart appeared to be different, morphological and histochemical… (More)
A comparison was made between collagenase-dispersed guinea-pig atrial and ventricular tissues. Heparin containing cells were stained with alcian blue at pH 2.2, and counted by an automated technique (Technicon H6000). The cells were challenged with the specific antigen (ovalbumin), with antisera to guinea-pig IgG (non-subclass specific), IgG1 and IgG2, and… (More)
We have studied the distribution of mast cells (MC) in different tissues of the guinea pig (GP), and certain aspects of their histochemical and cytochemical properties. In preparations fixed with Carnoy's fluid, MC were extremely well-preserved and were detected in all tissue sections and cytocentrifuge smears obtained from enzymically dispersed cells after… (More)
We report the direct demonstration of IgE-bearing mast cells in human heart and kidney, as shown by immunohistochemical methods. This finding lends further support to the role of mast cells and IgE in the direct involvement of these two organs in immediate-type allergic reactions.
By comparison with ventricular tissues, collagenase-dispersed cell suspensions obtained from atrial tissues of sensitized guinea-pigs showed a higher histamine content, a higher proportion of mast cells, and a higher release with antigen or antisera to IgG, IgG1 and IgG2 of the following mediators: histamine, thromboxane B2 and leukotriene C4.
Histamine was released from mast cells in isolated perfused heart and kidney of the rat, but not from mast cells in guinea-pig tissues, by a substance P (SP) analogue (SP(1-4)-NH-C12H25), SP(1-4)-C12 for abbreviation. This peptide also released histamine from peritoneal mast cells and basophil leucocytes of the rat. Substance P itself was compared with… (More)
This report suggests that the release of various inflammatory mediators such as histamine, LTC4, D4 and E4, and TXA2 measured as the stable metabolite TXB2 are partly responsible for the various cardiac and renal effects of the complement fragment C5a des Arg anaphylatoxin, in addition to its direct vasoconstrictor activity.
Background: Recently an adipose-tissue-derived protein termed visfatin was described in 2005. It was previously identified as a growth factor for early B-lymphocytes termed pre -B cell colony enhancing factor (PBEF). Visfatin was reported to be expressed almost exclusively in visceral adipose tissue and has insulin-like metabolic effects. These findings are… (More)