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It has recently been shown that large karyophilic proteins are transported across the nuclear envelope in amphibian oocytes. In consideration of this, the present experiments were performed to identify the specific sites within the envelope through which transport occurs and determine if molecular size is a limiting factor in the transport process. The(More)
The development of a competitive immunoassay for insulin that is based on capillary zone electrophoresis with laser-induced fluorescence detection (CZE-LIF) is described. The assay uses fluorescein isothiocyanate-derivatized insulin (FITC-insulin) as a tracer and the Fab fragment of an anti-insulin monoclonal antibody as the immunoreagent. Reproducibility(More)
The separation of a selected group of N-nitrosamino acids and their syn and anti conformers by high-performance liquid chromatography using an alpha-cyclodextrin bonded silica gel column and a mobile phase of acetonitrile-triethylammonium acetate was achieved. The effects of mobile phase pH and concentration of acetonitrile on resolution and elution times(More)
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