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To study the topology of Na+,K+-ATPase monoclonal antibodies (MAbs) specific for membrane-bound enzyme were produced. Using immunofluorescence staining of viable cells or smears of a pig kidney embryonic (PKE) cell line, two groups of MAbs were selected, namely those binding to extra- or intracellular portions of the alpha-subunit. The extracellular(More)
The interaction of three monoclonal antibodies with human spleen ferritin has been studied. Using titration of various monoclonal antibody-containing media with the immobilized antigen, the specific content of active antibodies capable of binding to ferritin was determined, which was 22-27% for ascitic fluids, 35-50% for total mouse IgG and 88% for(More)
New Delhi metallo-beta-lactamase (NDM-1) is a plasmid-borne enzyme conferring bacterial resistance to any known beta-lactam antibiotics. There is urgent need to develop efficient NDM-1 inhibitors and approaches for early diagnostics of NDM-1 that necessitates structural studies of the enzyme and analysis of the secretion pathways and localization of the(More)
Monoclonal antibodies (MCAs) to the E. coli O157: H7 O-antigen characterized by a high level of activity and specificity have been obtained. We investigated their biochemical properties and diagnostic importance. Based on these results, we constructed a latex assay using monoclonal antibodies for the identification of enterohemorrhagic E. coli belonging to(More)
Three monoclonal antibodies to human spleen ferritin were produced and their interaction with soluble and immobilized ferritins studied. An immunoassay was developed to monitor the interaction of soluble biotinylated ferritin and the antibody with subsequent separation of the soluble complexes on streptavidin-cellulose. Analysis of immunoreactivities of a(More)
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