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The vitality of freshly isolated and of cryopreserved (-1 degree C/min, 10% DMSO) islets of the rat pancreas was determined by means of the colorimetric MTT-test. The recover rate of vital islet cells amounted to 35% after cryopreservation. The in vitro results were confirmed by the effects after syngeneic intraportal transplantation. The transplantation of(More)
Preservation of B-cells, following organ collection and cold ischaemia, was defined on 19 donor organs by means of histological tests and testing for insulin content in pancreatic tissue. The degree of preservation declined by 50 per cent after two hours of cold ischaemia. The Velcro technique and uncrushed organ digestion were used for isolation. Islet(More)
Nine of 38 islet isolation experiments, using the duct collagenase technique, were selected for quality checks on isolated islet tissue. Pancreas was harvested, following aortic multi-organ perfusion. The total number of islets isolated amounted to 112,461 +/- 11,828 in 13.7 ml of suspension on average. In vitro secretion of beta cells was increased by a(More)
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