N. B. Gamaleya

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By means of two different types of enzyme-linked immunosorbent assay (ELISA) techniques, antibodies to methadone were detected in blood plasma of heroin addicts on methadone maintenance treatment. In 11-15% of cases immunoglobulin (Ig) M antibodies were detected, while IgG antibodies were observed in 33-40%. At least two types of antibodies to methadone(More)
In the early 1970's, some papers appeared reporting an immune response to opiates in animals treated with morphine and in heroin addicts, but further researches failed to confirm these results in humans. The aim of the present work is investigating with a newly developed enzyme immunoassay the existence of specific antibodies to morphine in a group of(More)
An ELISA method for detection of specific antibodies to morphine-protein conjugate (MPC) in humans and animals was elaborated. In humans, antibodies could be detected predominantly as the IgM class of immunoglobulins. Elevated levels of such antibodies were found in about 50% of opiate addicts and morphine-treated rats. In the case of prolonged and regular(More)
The phenomenon of induction of antibodies to morphine in morphine-treated animals and opiate addicts was shown by means of ELISA. The reaction of the immune system to injections of a morphine solution had the characteristics of a primary immune response. Opiate addicts differed from normal controls in the level of antibodies to morphine of IgM class.(More)
Chronic intoxication of rats with morphine during 2- and 4-week periods resulted in the emergence of antimorphine antibodies in the blood of 50 and 80% of the animals, respectively, in hemagglutination titers 1∶20 and higher. Antibodies were not detected in the control animals (not treated with morphine) but were detected in 17% of rats given alcohol during(More)
Chronic morphine treatment of rats for 2 and 4 weeks led to an increase in morphine-binding cells in the spleen, despite the general reduction of mononuclear cell content in the spleen and thymus. Simultaneously, serum antibodies to morphine (AbM) in haemagglutination titres 1:20 and higher appeared in 50% and 80% of animals, respectively. Animals with high(More)
The effect of morphine on adenylate cyclase activity in lymphocytes was tested in 20 normal controls, 16 alcoholics in withdrawal and 9 sober alcoholics. Alcoholics in withdrawal were characterized by a significantly increased stimulatory effect of morphine, whereas sober alcoholics showed an inhibitory effect. The morphine effect was abolished by naloxone(More)
The content of neuromediators, such as biogenic amines and their metabolites in the blood and urine was determined by spectrofluorometric methods in 14 patients with paranoid and 11 patients with simple schizophrenia (5 of them showing the apatho-abulic and 6 the psychopathlike syndromes), as well as in 12 healthy subjects who served as control. Both the(More)
According to data in the literature, in animal experiments morphine has an inhibitory action on the cellular and humoral immune response [5, 12]. On the other hand, a number of investigations have shown that chronic morphine poisoning induces the appearance of antibodies capable of binding specifically with radioactively labeled morphine in the blood of(More)
It is known that the serotonin and catecholamine systems of the organism take part in the realization of the morphine effect [1,2,5]. Morphine changes the metabolism of neurotransmitters such as dopamine (DA), norepinephrine (NE), and serotonin (5-HT) [6,8], raises the levels of endogenous tryptophan and 5-oxyindoleacetic acid [13], and accelerates the(More)