N A Bogoliubova

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Within the framework of studying the "2-cell block in vitro" phenomenon, two variants of inhibitory-induced stoppage of development at a two-cell stage were produced and analysed. Mimosine arrested the cleavage on the G1/S interface, and genistein at G2 stage of the second cell cycle. In the experimentally blocked embryos a detailed study was made of the(More)
The embryos from many outbred and inbred strains of mice are arrested at the late 2-cell stage when cultured in vitro in simple culture media. This phenomenon is referred to as the "2-cell block in vitro". The ultrastructural morphology of the nuclei of the blocked embryos is not yet well described. In the present paper we documented the results of a(More)
Mouse embryos were analyzed vitally, on fixed total and chromosome preparation at the two-cell stage and during transition to the four-cell stage, upon polyethylene glycol-induced fusion of sister blastomeres and early cleavage of thus obtained somatic hybrids. Vital staining by Rhodamine 123 showed a diffuse distribution of functionally active mitochondria(More)
In this study, preimplantation mouse embryos were used as a new model for investigation of actin distribution in the nuclei and identification of functional forms of intranuclear actin. Combination of direct detection of actin by fluorescent-conjugated falloidin and DNase I with the method of indirect immunofluorescence was applied as an integrated approach(More)
Changes in the distribution of mitochondria in the two-cell mouse embryos preceding the developmental arrest in vitro, caused by a genetically determined "two-cell block in vitro" or genisteine treatment, were examined vitally using the mitochondrial-specific probe rhodamine 123 and conventional fluorescence microscopy. In the former case, serious(More)
The capacity of sister blastomeres of mouse embryos for induced fusion changed during the 2-cell stage. It was at low level (24%) at the early 2-cell stage, increased and reached 98.5% at the middle 2-cell stage and fell sharply to 31% at the late 2-cell stage. At the time corresponding to the G2/M-phase of the cell cycle the blastomeres fused in only 8% of(More)
Capacity to the physical work and functional status were studied during the prolonged (1 year) work in the mountainous conditions (3,600 m above sea level). The fatigue grade and capacity level were proved to depend on individual adaptation to the mountainous conditions.