Structural characterization of toxic oligomers that are kinetically trapped during α-synuclein fibril formation
- Serene W. Chen, S. Drakulić, N. Cremades
- BiologyProceedings of the National Academy of Sciences
- 8 April 2015
The findings reveal the inherent multiplicity of the process of protein misfolding and the key role the β-sheet geometry acquired in the early stages of the self-assembly process plays in dictating the kinetic stability and the pathological nature of individual oligomeric species.
Chemical properties of lipids strongly affect the kinetics of the membrane-induced aggregation of α-synuclein
- C. Galvagnion, James W. P. Brown, C. Dobson
- BiologyProceedings of the National Academy of Sciences
- 13 June 2016
The results show that the chemical properties of lipids determine whether or not the lipids can trigger the aggregation of α-synuclein, thus affecting the balance between functional and aberrant behavior of the protein.
Conserved Amphipathic Helices Mediate Lipid Droplet Targeting of Perilipins 1–3
- Emily R. Rowe, M. Mimmack, D. Savage
- Biology, ChemistryJournal of Biological Chemistry
- 7 January 2016
It is concluded that highly conserved 11-mer repeat regions of PLINs target lipid droplets by folding into AHs on the droplet surface, thus enabling PLIns to regulate the interface between the hydrophobic lipid core and its surrounding hydrophilic environment.
Effect of the interplay between protein and surface on the properties of adsorbed protein layers
- Myriam M. Ouberai, Kairuo Xu, M. Welland
- Materials ScienceBiomaterials
- 1 August 2014
Cisplatin-tethered gold nanospheres for multimodal chemo-radiotherapy of glioblastoma.
- Sonali Setua, Myriam M. Ouberai, S. Piccirillo, C. Watts, M. Welland
- Biology, PhysicsNanoscale
- 21 August 2014
The first successful chemo-radiotherapy on patient derived treatment resistant GBM cells using a cisplatin-tethered gold nanosphere and photo/Auger electron mediated radiosensitisation leading to complete ablation of the tumour cells in an in vitro model system is reported.
A comprehensive study of lysozyme adsorption using dual polarization interferometry and quartz crystal microbalance with dissipation.
- Kairuo Xu, Myriam M. Ouberai, M. Welland
- Chemistry, PhysicsBiomaterials
- 1 February 2013
α-Synuclein Senses Lipid Packing Defects and Induces Lateral Expansion of Lipids Leading to Membrane Remodeling*
- Myriam M. Ouberai, Juan Wang, M. Welland
- Biology, ChemistryJournal of Biological Chemistry
- 5 June 2013
The ability of α-Syn to sense lipid packing defects and to remodel membrane structure supports its proposed role in vesicle trafficking.
Synthesis and biological evaluation of clicked curcumin and clicked KLVFFA conjugates as inhibitors of beta-amyloid fibril formation.
- Myriam M. Ouberai, P. Dumy, S. Chierici, Julian Garcia
- Biology, ChemistryBioconjugate chemistry
- 12 October 2009
This work highlights the value of combining Abeta-recognition domains with a steric hindrance-inducing scaffold for preventing amyloid fibril formation.
A Multimeric Quinacrine Conjugate as a Potential Inhibitor of Alzheimer's β‐Amyloid Fibril Formation
- G. Dolphin, S. Chierici, Myriam M. Ouberai, P. Dumy, Julian Garcia
- Chemistry, BiologyChemBioChem
- 14 April 2008
This work suggests that assembling multiple copies of acridine moieties to a central scaffold, for multiple interactions, is a promising strategy for the engineering of inhibitors against Aβ fibril formation.
A Simple Bioconjugate Attachment Protocol for Use in Single Molecule Force Spectroscopy Experiments Based on Mixed Self-Assembled Monolayers
- S. Attwood, Anna M. C. Simpson, M. Welland
- Chemistry, BiologyInternational Journal of Molecular Sciences
- 19 October 2012
A novel approach of mixed self-assembled monolayers of alkanethiols in conjunction with a heterobifunctional crosslinker is utilised for single molecule force spectroscopy, demonstrating the effectiveness of the protocol by probing the biotin-avidin interaction.
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