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After incorporation of the base analogue 5-bromodeoxyuridine (BUdR) into PHA activated human lymphocyte cultures and subsequent staining with the benzimidazol compound “33258 Hoechst” and with Giemsa stain, the sister chromatids of metaphase chromosomes are stained differently in the 2nd and 3rd cycle of cell division. The first mitosis appears after(More)
We have studied the distribution of mGluR2/3 in the mouse superior colliculus (SC) with antibody immunocytochemistry and the effect of enucleation on this distribution. We also compared this labeling to that for calbindin D28K. Anti-mGluR2/3-immunoreactive (IR) cells formed distinctive laminar patterns within the lower optic and upper intermediate gray(More)
Lymphocyte proliferation and nucleoid sedimentation were studied in a patient with premature aging resembling the Werner's syndrome (WS). Onset of patchy brown hyperpigmentations at the age of 9 months permitted distinction from classical WS and suggested a WS-like premature aging disease. By photometric recording of densitiy changes during cell culture, we(More)
Characteristic patterns of constitutive heterochromatin in human metaphase chromosomes are shown by treatment with enzymes, variation of incubation time in standard saline citrate solution and staining with fluorochrome and Giemsa. The results obtained in these studies are summarized as follows: 1. DNase persistant regions of metaphase chromosomes are shown(More)
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