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A series of titania-chitosan nanocomposites (2:x (0.12, 0.25, 0.5, 1.0 and 2.0g)) were synthesized using in situ sol-gel method and comprehensively characterized using conventional techniques. The resultant particles showed anatase phase, spherical and irregular morphology with particle size of 4.5-10.5nm. Nanocomposites with higher surface area(More)
Both conventional and real time PCR (rt-PCR) assays based on the amplification of a 103bp fragment from the DNA polymerase (DNA pol) gene (conserved, non-structural) of Orf virus (ORFV) were developed for detection and semi-quantitation of ORFV DNA from infected cell culture and clinical samples. The latter technique was based on TaqMan chemistry. The(More)
In this study, thermostability of a Vero cell attenuated live camelpox vaccine under conventional lyophilization conditions has been evaluated. Three stabilizers were used separately for freeze-drying the vaccine and the stability of the vaccine, both in freeze-dried and reconstituted forms at different temperatures was assessed. The study revealed that the(More)
The study describes the development of TaqMan hydrolysis probe based real time PCR (rt-PCR) assay targeting the ankyrin repeat protein (C18L) gene sequences for the detection and quantitation of camelpox virus (CMLV) nucleic acid and its comparison with established conventional and SYBR green rt-PCR assays. The assay was specific with an efficiency of(More)
In this study, silver-doped silica- and phosphate-based nanobioactive glass compositions (58SiO2-(33- x)CaO-9P2O5-xAg2O) (x = 0, 0.5, 1, 2 and 3 mol%) were synthesised by a simple and cost-effective sol-gel method. The prepared samples were characterised by X-ray diffraction, Fourier transform infrared spectroscopy, transmission electron microscopy,(More)
In the present study, two sheeppox vaccines made from strains [sheeppox virus-Srinagar (SPPV-Srin) and Ranipet (SPPV-R)] indigenous to India and adapted to Vero cells were compared in terms of their safety, potency, efficacy and antigenic value with the commercial in-use Roumanian Fanar (SPPV-RF) vaccine, a foreign strain adapted in primary lamb testes(More)
A wet chemical approach was used to synthesis HAp-chitosan-gelatin nanocomposites at different wt.% of chitosan-gelatin (CG) ratios such as CG-00, CG-40, CG-31, CG-22, CG-13 and CG-04. XRD analysis confirms the formation of HAp on nanocomposite matrix and the decreased crystallite size was found with the decrease in chitosan and increase in gelatin(More)
In this investigation, ultrasonication process was used for the synthesis of magnesium doped nano-hydroxyapatite (MH) (0, 1, 2, and 3 mol% of Mg concentration) particles with controlled size and surface morphology. The size of the prepared MH particles was in the range of 20-100 nm with narrow distribution. Increase in the concentration of Mg reduced the(More)
In the present study, SYBR Green and TaqMan real time PCRs (rt-PCR) based on the C18L gene (encodes ankyrin repeat protein) of camelpox (CMLV) and buffalopox viruses (BPXV) were, respectively employed for potency evaluation of live attenuated camelpox and buffalopox vaccines. Cells infected with the respective vaccine viruses were harvested at critical time(More)
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