Muriel van Schilfgaarde

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BACKGROUND The procoagulant properties of microparticles (MPs) are due to the of the presence of phosphatidylserine (PS) and tissue factor (TF) on their surface. The latter has been demonstrated especially on MPs derived from monocytes. OBJECTIVES To investigate the relative contribution of TF and factor (F)XII in initiating coagulation on MPs derived(More)
INTRODUCTION Microparticles (MP) can be elevated in cancer and thromboembolic disease. We hypothesized a role for MP in the hypercoagulable state in breast cancer patients using endocrine therapy, in whom both cancer and the use of endocrine therapy are independent risk factors for the development of thrombosis. DESIGN AND METHODS Plasma samples were(More)
INTRODUCTION Renal insufficiency increases the half-life of low molecular weight heparins (LMWHs). Whether continuous venovenous hemofiltration (CVVH) removes LMWHs is unsettled. We studied hemostasis during nadroparin anticoagulation for CVVH, and explored the implication of the endogenous thrombin potential (ETP). METHODS This cross-over study,(More)
The sequenced genomes of pathogenic Neisseria meningitidis strains contain up to eight genes putatively encoding autotransporters, which are secreted proteins implicated in virulence. Here, we have characterized one of these genes, designated ausI, which encodes an autotransporter of the serine protease family. It was found to be specific for N.(More)
Nonencapsulated Haemophilus influenzae frequently persists in the lungs of chronic obstructive pulmonary disease (COPD) and cystic fibrosis (CF) patients for prolonged periods of time. The bacteria are not eradicated by antibiotic treatment of the patients or by specific antibodies that are found in the sputum and sera of these patients. We investigated(More)
Haemophilus influenzae penetrates the respiratory epithelium during carriage and invasive disease, including respiratory tract infections. We developed an in vitro model system consisting of lung epithelial NCI-H292 cells on permeable supports to study the passage of H. influenzae through lung epithelial cell layers. The NCI-H292 cells formed tight layers(More)
BACKGROUND Entry criteria included patients who developed sinusoidal obstruction syndrome (SOS) at a single centre from January 2000 to December 2011. Patients who underwent haemopoietic stem cell transplantation or actinomicyn-based chemotherapy for nephroblastoma were selected. The study group comprised five patients with SOS who were compared with a(More)
M. C . V AN AALDEREN ,* 1 M. C . T RAPP ENB URG,* 1 M. VA N SCHILF GA ARDE , P . J . MOLENAAR , H. TEN C ATE , W. E . TERPSTRA* and A . LEY TE *Department of Internal Medicine, and Department of Clinical Chemistry, Onze Lieve Vrouwe Gasthuis, Amsterdam; and Department of Internal Medicine and Cardiovascular Research Institute Maastricht, Maastricht(More)
Haemophilus influenzae penetrates between epithelial cells via an unknown mechanism. A chromosomal library of nonencapsulated H. influenzae strain A960053 DNA was constructed in Escherichia coli DH5alpha to identify bacterial genes contributing to this paracytosis. Two E. coli clones that contained open reading frames (ORFs) homologous to HI0636 to HI0641(More)
Microparticles (MP) may be generated during the in vitro storage of red blood cells but also in certain clinical conditions1. Grisendi G. and her colleagues from the University of Modena and Reggio Emilia present a new multiparametric staining assay for detecting red blood cell microparticles (RMP) from human red blood cells in vitro using flow cytometry2.(More)