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Preharvest sprouting limits the consistent production of high quality wheat in many regions of the world. Improvements in tolerance from the introduction of better grain dormancy at, or near, harvest-ripeness would be expected to have a significant impact on the incidence and severity of sprouting. Genetic and molecular investigations have provided new(More)
Genetic relationships of the complex of Fusarium species associated with crown rot and head blight in cereals and some species associated with plant diseases in general were examined by distance and maximum parsimony algorithms of their internal transcribed spacer sequences. The analysis clustered the complex of Fusarium species that causes root and crown(More)
A molecular assay with enhanced specificity and sensitivity has been developed to assist in the surveillance of Karnal bunt, a quarantineable disease with a significant impact on international trade. The protocol involves the release of DNA from spores, PCR amplification to enrich Tilletia-specific templates from released DNA and a five-plex, real-time PCR(More)
Tilletia indica, the cause of Karnal bunt of wheat, is the target of strict quarantine regulations by most wheatgrowing countries and its presence raises trade barriers to wheat exports. The international diagnostic protocol currently used in Australia involves the tentative identification of the spores based on morphology followed by germination of the(More)
The current surveillance protocol for Karnal bunt of wheat in most countries, including the USA, European Union (EU), and Australia, involves the tentative identification of the spores based on morphology followed by a molecular analysis. Germination of spores is required for confirmation which incurs a delay of about two weeks, which is highly(More)
This study has found numerous novel genetic variants of GA-insensitive dwarfing genes with potential agricultural value for crop improvement. The cultivar, Spica is a tall genotype and possesses the wild-type genes of Rht-A1a, Rht-B1a and Rht-D1a. The cultivar Quarrion possesses a null mutant in the DELLA motif in each of the 3 genomes. This is a first(More)
Using de novo assembly of 46 million paired end sequence reads of length 250 bp for a myrtle rust isolate, we have estimated its genome size to be between 103 and 145 Mb and the number of proteins as >19,000. Annotation of the contigs found a very large percentage of proteins are associated with molecular functions of DNA binding or binding in biological(More)
The HfrDrd gene has been reported to be induced specifically in the wheat’s defence response to Hessian fly larvae and is not a general stress-responsive gene. The gene is located on chromosome 7DS with a homeologue on 7AS. In this study, two SNPs in the gene that result in amino acid substitutions in the dirigent-like protein have been identified. Both are(More)
Tilletia indica, the cause of Karnal bunt of wheat, replaces part of the seed with a black powdery mass containing millions of spores and produces a strong unpleasant odour like rotten fish. The fishy smell has serious consequences for the marketability of wheat. The fungus is thus subjected to very strict quarantine regulations in countries not known to(More)
Tilletia indica causes the disease Karnal bunt in wheat. The disease is under international quarantine regulations. Comparative mitochondrial (mt) genome analysis of T. indica (KX394364 and DQ993184) and T. walkeri (EF536375) has found 325 to 328 SNPs, 57 to 60 short InDels (from 1 to 13 nt), two InDels (30 and 61 nt) and five (>200 nt) presence/absence(More)
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