Burkholderia mallei (Bm), the causative agent of the predominately equine disease glanders, is a genetically uniform species that is very closely related to the much more diverse species Burkholderia pseudomallei (Bp), an opportunistic human pathogen and the primary cause of melioidosis. To gain insight into the relative lack of genetic diversity within Bm,… (More)
We collected epidemiologic and molecular data from Burkholderia mallei isolates from equines in Punjab, Pakistan from 1999 through 2007. We show that recent outbreaks are genetically distinct from available whole genome sequences and that these genotypes are persistent and ubiquitous in Punjab, probably due to human-mediated movement of equines.
BACKGROUND Glanders is a contagious and fatal zoonotic disease of solipeds caused by the Gram-negative bacterium Burkholderia (B.) mallei. Although regulations call for culling of diseased animals, certain situations e.g. wild life conservation, highly valuable breeding stock, could benefit from effective treatment schemes and post-exposure prophylaxis. … (More)
BACKGROUND The in vivo diagnosis of glanders relies on the highly sensitive complement fixation test (CFT). Frequently observed false positive results are troublesome for veterinary authorities and cause financial losses to animal owners. Consequently, there is an urgent need to develop a test with high specificity. Hence, a Western blot assay making use of… (More)
A new hepatitis E virus (HEV-7) was recently found in dromedaries and 1 human from the United Arab Emirates. We screened 2,438 dromedary samples from Pakistan, the United Arab Emirates, and 4 African countries. HEV-7 is long established, diversified and geographically widespread. Dromedaries may constitute a neglected source of zoonotic HEV infections.
In Computer Network number of security techniques provide security consolation but not up to optimal security extent. There are massive attacks and efficient viruses travel across the network which incapacitates computer system and default configuration of the operating system. Preceding techniques can capture anomalous activates and previous known attacks… (More)
A Data Warehouse is a computer system designed for archiving and analyzing an organization's historical data, such as sales, customers, products, salaries, or other information from day-today operations OLTP. Normally, an organization summarizes and copies information from its operational systems to the data warehouse on a regular schedule, such as daily,… (More)
In the present study, a fatal case caused by honeybee (Apis cerana) stings was documented in a female German shepherd dog that was presented at the Veterinary Teaching Hospital, University of Agriculture Faisalabad, Pakistan. Characteristic clinical signs included hematuria, hematemesis, incoordination and convulsions along with evidence of massive honeybee… (More)
— The Real time locating system (RTLS) determines and tracks the location of assets and people. This paper presents a novel application to estimate the position and velocity of vehicle using wireless sensor network. Two Anchor nodes are used as reader along roadside and total distance between them is known. Whenever a moving vehicle with tag comes in… (More)
Fuzzy relation equations are becoming extremely important in order to investigate the optimal solution of the inverse problem even though there is a restrictive condition for the availability of the solution of such inverse problems. We discussed the methods for finding the optimal (maximum and minimum) solution of inverse problem of fuzzy relation equation… (More)