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alpha-Latrotoxin (LTX) stimulates massive exocytosis of synaptic vesicles and may help to elucidate the mechanism of regulation of neurosecretion. We have recently isolated latrophilin, the synaptic Ca2+-independent LTX receptor. Now we demonstrate that latrophilin is a novel member of the secretin family of G protein-coupled receptors that are involved in(More)
Pure alpha-latrotoxin is very inefficient at forming channels/pores in artificial lipid bilayers or in the plasma membrane of non-secretory cells. However, the toxin induces pores efficiently in COS-7 cells transfected with the heptahelical receptor latrophilin or the monotopic receptor neurexin. Signaling-deficient (truncated) mutants of latrophilin and(More)
We report here the first three-dimensional structure of alpha-latrotoxin, a black widow spider neurotoxin, which forms membrane pores and stimulates secretion in the presence of divalent cations. We discovered that alpha-latrotoxin exists in two oligomeric forms: it is dimeric in EDTA but forms tetramers in the presence of Ca2+ or Mg2+. The dimer and(More)
Computer network technologies have been growing explosively and the study in computer networks is being a challenging task. To make this task easy, different users, researchers and companies have developed different network modelling and simulation (MS) tools. These network MS tools can be used in education and research as well as practical purposes. They(More)
In present scenario when the term fraud comes into a discussion, credit card fraud clicks to mind so far. With the great increase in credit card transactions, credit card fraud has increasing excessively in recent years. Fraud detection includes monitoring of the spending behavior of users/ customers in order to determination, detection, or avoidance of(More)
alpha-latrotoxin (LTX) stimulates massive release of neurotransmitters by binding to a heptahelical transmembrane protein, latrophilin. Our experiments demonstrate that latrophilin is a G-protein-coupled receptor that specifically associates with heterotrimeric G proteins. The latrophilin-G protein complex is very stable in the presence of GDP but(More)
The complete Serine 8-type gonadotropin releasing hormone (GnRH) coding sequence with a substantial 5-prime regulatory sequence (5 kb) has been isolated and characterised in Nile Tilapia (Oreochromis niloticus) from a relevant genomic library. The primary structure of the protein precursor was identified for this gene. The promoter efficacy has been tested(More)
A novel procedure of alpha-latrotoxin (alpha LTX) purification has been developed. Pure alpha LTX has been demonstrated to exist as a very stable homodimer. Such dimers further assemble into tetramers, and Ca(2+), Mg(2+) or higher toxin concentrations facilitate this process. However, when the venom is treated with EDTA, purified alpha LTX loses the ability(More)
Internet is the main tool for e-business. E-transaction is made faster by Internet. With the increase of e-transaction internet fraud or e-business fraud is increasing. Credit fraud in the banking sector is a growing concern. Few sort of card (debit/credit) fraud is decreasing by providing detection and prevention system from banks and government. But(More)
Achieving a deep understanding of the Internet topology has proved very challenging since it involves solving difficult task, e.g. mapping the actual topology, characterising it and developing generation models that capture the fundamental properties. There are two categories of tools which were developed to deal with network topology, namely, topol-ogy(More)