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We developed a novel large-scale micropropagation pathway for date palm (Phoenix dactylifera L.) based on organogenesis. We obtained organogenic stems from shoot tip explants of the Moroccan date palm cultivar Najda, and investigated shoot proliferation from these organogenic stems in vitro on various media; Beauchesne medium (BM) and Murashige and Skoog(More)
We investigated the effects of cytokinins and the physical state of the culture medium on in vitro shoot multiplication of date palm (Phoenix dactylifera L.) cv. Boufeggous. Organogenic cultures obtained from shoot tip explants were cultured on stationary liquid or semi-solid half-strength Murashige and Skoog medium (MS/2) supplemented with 3 μM(More)
The present study aimed to determine the best combination of plant growth regulators (PGRs) and light intensity in order to improve organogenesis of date palm (Phoenix dactylifera L.) cultivar Mejhoul. Organogenic cultures obtained from shoot tip explants were cultured on half-strength Murashige and Skoog (MS/2) medium supplemented with various combinations(More)
The effects of major mineral salts, L-glutamine, myo-inositol and carbon source on shoot bud proliferation of date palm (Phoenix dactylifera L.) cv. Mejhoul were evaluated. Different concentrations of ammonium nitrate (NH4NO3; 550, 825 or 1650 mg/L), potassium nitrate (KNO3; 633.3, 950 or 1900 mg/L), calcium chloride dehydrate (CaCl2·2H2O; 147, 220 or 440(More)
An efficient regeneration system through somatic embryogenesis was developed for date palm cv. Najda. Adventitious bud and proximal leaf segments cultured on Murashige and Skoog (MS) medium supplemented with various combinations of auxins and cytokinins induced embryogenesis after at least 6 months of culture. Somatic embryogenesis induction seemed(More)
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