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To construct an efficient lipid-containing wastewater treatment system, microorganisms that degrade lipids efficiently were isolated from various environmental sources. Strain DW2-1 showed the highest rate of degradation of 1% (w/v) salad oil among the isolated strains. Strain DW2-1 was identified as Burkholderia sp. and designated Burkholderia sp. DW2-1.(More)
The degradation of alkylcyclohexane by Acinetobacter sp. ODDK71 was investigated. Strain ODDK71 degraded alkylcyclohexanes (alkyl side chain length of > or = 12) by co-metabolism when hexadecane was used as a growth substrate. GGMS analysis of co-metabolized products from dodecylcyclohexane suggests that strain ODDK71 degraded dodecylcyclohexane via a ring(More)
Two microorganisms (NDKK48 and NDKY76A) that degrade long-chain cyclic alkanes (c-alkanes) were isolated from soil samples. Strains NDKK48 and NDKY76A were identified as Rhodococcus sp. and Gordonia sp., respectively. Both strains used not only normal alkane (n-alkane) but also c-alkane as a sole carbon and energy source, and the strains degraded more than(More)
Accumulations of inorganic nitrogen (NH₄⁺, NO₂⁻, and NO₃⁻) were analyzed to evaluate the nitrogen circulation activity in 76 agricultural soils. Accumulation of NH₄⁺ was observed, and the reaction of NH₄⁺→ NO₂⁻ appeared to be slower than that of NO₂⁻ → NO₃⁻ in agricultural soil. Two autotrophic and five heterotrophic ammonia-oxidizing bacteria (AOB) were(More)
The relationship between the autodegradation and thermostability of thermolysin (TLN) was studied. Four autodegradation sites in TLN were identified in the presence of Ca(2+). One of the sites was identified as Gly(154)-Leu(155), and Leu(155) was substituted with various amino acids, X = Ala, Ser, Phe, and Gly, by site-directed mutagenesis. The(More)
Bluegill-degrading bacteria were isolated from various environmental sources. Brevibacillus sp. BGM1 degraded bluegill efficiently at 50 degrees C, and its culture supernatant showed the highest peptide and amino acid concentrations as trichloroacetic acid (TCA) soluble fraction (ASF) (10.7 mg/ml) of all supernatants obtained with bluegill as a substrate.(More)
The growth of Brassica campestris, Solanum tuberosum L., Lycopersicum esculentum and Brassica junce in the field were promoted by the degraded soybean meal products (DSP). The root hair number of Brassica campestris was increased when 10 μl DSP (containing 30 mg peptides + amino acids ml−1) were added to 10 ml plant growth medium. A chemical fertilizer and(More)
A real-time PCR quantification method for indigenous hydrocarbon-degrading bacteria (HDB) carrying the alkB gene in the soil environment was developed to investigate their distribution in soil. The detection limit of indigenous HDB by the method was 1 × 10(6) cells/g-soil. The indigenous HDB were widely distributed throughout the soil environment and ranged(More)
BACKGROUND Excess visceral adipose tissue (VAT) is closely associated with the presence of coronary artery plaques that are vulnerable to rupture. Patients with diabetes mellitus (DM) have more VAT than patients without DM, but the extent to which VAT contributes to the characteristics of coronary plaques before and after the development of DM is not fully(More)
The degradation pathways for cyclic alkanes (c-alkanes) in Rhodococcus sp. NDKK48 were investigated. Strain NDKK48 used dodecylcyclohexane as a sole carbon and energy source, and five metabolites in the dodecylcyclohexane degradation pathway were detected by gas-chromatography/mass spectra. The metabolites were identified as cyclohexanecarboxylic acid,(More)