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Reviewers The Publications Committee of the American Physiological Society gratefully acknowledges the services of the following reviewers who assisted the Editorial Board in the reviews of manuscripts. I would like to recommend that our library subscribe to the American Journal of Physiology–Cell Physiology. This is a journal published online and in print(More)
Recognition of sweet, bitter and umami tastes requires the non-vesicular release from taste bud cells of ATP, which acts as a neurotransmitter to activate afferent neural gustatory pathways. However, how ATP is released to fulfil this function is not fully understood. Here we show that calcium homeostasis modulator 1 (CALHM1), a voltage-gated ion channel,(More)
PURPOSE To develop a reliable technique for measuring intraocular pressure (IOP) in the mouse. METHODS An electrophysiologic approach-the servo-null micropipette system (SNMS)-for measuring hydrostatic pressure was adapted for the mouse eye. Fine-tipped (5 microm in diameter) micropipettes were advanced across the cornea with a piezoelectric(More)
Anisosmotic cell swelling triggers a regulatory volume decrease (RVD) in cell lines derived from human nonpigmented ciliary epithelium. Measurements of cell volume have indicated that the RVD reflects activation of K+ and/or Cl- channels. We have begun to characterize the putative channels by whole cell patch clamping. The results obtained by altering the(More)
PURPOSE To determine whether the Na+-K+-2Cl- symport or the parallel Na+/H+ and Cl-/HCO3- antiports provide the dominant pathway for NaCl uptake into the ciliary epithelium. Both pathways are known to support NaCl entry from the stroma into the pigmented ciliary epithelial (PE) cells, after which Na+ and Cl- diffuse across the gap junctions into the(More)
PURPOSE To test the putative role of A(3) adenosine receptors (ARs) in modulating intraocular pressure (IOP). METHODS IOP was monitored for up to 32 minutes in A3-knockout (A3AR-/-) and A3AR+/+ control mice by the servo-null approach. The IOP responses to adenosine, A3AR agonists and A3AR antagonists were studied singly or in combination in both strains.(More)
The aqueous humor is secreted by the ciliary epithelium, a bilayered syncytial epithelium comprising a pigmented (PE) cell layer abutting the stroma and a nonpigmented (NPE) cell layer facing the aqueous phase. As in other epithelia, secretion depends on the transfer of solute, with water passively following. Na+, K+, and Cl- enter the syncytium principally(More)
PURPOSE To test whether blocking the Na+/H+ antiport reduces intraocular pressure (IOP) in the mouse. METHODS The electrophysiologic approach (the servo-null micropipette system, SNMS) that had been adapted for continuously monitoring IOP in the mouse was used in a study of the effects of a series of transport inhibitors. RESULTS Topical application of(More)
PURPOSE To determine the potential role of ClC-3, a PKC-inhibitable Cl(-) channel, in mediating the swelling-activated Cl(-) current (I(Cl,swell)) of native bovine nonpigmented ciliary epithelial (NPE) cells. METHODS Native bovine NPE cells were freshly harvested by enzymatic digestion. Whole-cell currents were recorded by patch-clamp measurements either(More)
PURPOSE The authors identify the signaling pathways for the regulatory volume decrease (RVD) of nonpigmented ciliary epithelial (NPE) cells. The RVD is a regulatory response triggered by swelling and reflecting KCl release by NPE cells. METHODS The cell volumes of human nonpigmented ciliary epithelial cells were measured in suspension by electronic cell(More)