Monica Zoppè

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The transactivator protein of human immunodeficiency virus type 1 Tat has the unique property of mediating the delivery of large protein cargoes into the cells when present in the extracellular milieu. Here we show that Tat fusion proteins are internalized by the cells through a temperature-dependent endocytic pathway that originates from cell membrane(More)
In living cells, proteins are in continuous motion and interaction with the surrounding medium and/or other proteins and ligands. These interactions are mediated by protein features such as electrostatic and lipophilic potentials. The availability of protein structures enables the study of their surfaces and surface characteristics, based on atomic(More)
This work presents a technical solution for the creation of visualization schemes for biological data on the web platform. The proposed technology tries to overcome the standard approach of molecular/biochemical visualization tools, which generally provide a fixed set of visualization methods. This goal is reached by exploiting the capabilities of the WebGL(More)
In this and the associated article BioBlender: Fast and Efficient All Atom Morphing of Proteins Using Blender Game Engine, [1] we present BioBlender, a complete instrument for the elaboration of motion [1] and the visualization (here) of proteins and other macromolecules, using instruments of computer graphics. The availability of protein structures enables(More)
Human cytomegalovirus (HCMV) encodes a protein related to the large (R1) subunit of ribonucleotide reductase (RR), but does not encode the corresponding small (R2) subunit. The R1 homologue, UL45, lacks many catalytic residues, and its impact on deoxyribonucleotide (dNTP) production remains unknown. Here, UL45 is shown to accumulate at late stages of(More)
Suicide gene therapy using herpes simplex virus type-1 (HSV-1) thymidine kinase (TK) is a widely exploited approach for gene therapy of cancer and other hyperproliferative disorders. Despite its popularity, clinical success has been so far hampered mostly by the relative inefficiency of TK gene transfer and its limited bystander effect. Here we report that(More)
When exogenous DNA is stably introduced in mammalian cells, it is typically integrated in random positions, and only a minor fraction enters a pathway of homologous recombination (HR). The complex Rad51/Rad52 is a major player in the management of exogenous DNA in eukaryotic organisms and plays a critical role in the choice of repair system. In(More)
In this and the associated article BioBlender: A Software for Intuitive Representation of Surface Properties of Biomolecules [1], we present BioBlender as a complete instrument for the elaboration of motion (here) and the visualization [1] of proteins and other macromolecules, using instruments of computer graphics. A vast number of protein (if not most)(More)
Up-regulation of Ca2+ entry through Ca2+ channels by high rates of beating is involved in the frequency-dependent regulation of contractility: this process is crucial in adaptation to exercise and stress and is universally known as force-frequency relation (FFR). Disturbances in calcium handling play a central role in the disturbed contractile function in(More)
The knowledge of the spatial organisation of the chromatin fibre in cell nuclei helps researchers to understand the nuclear machinery that regulates dna activity. Recent experimental techniques of the type Chromosome Conformation Capture (3c, or similar) provide high-resolution, high-throughput data consisting in the number of times any possible pair of dna(More)