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Programmed cell death occurs naturally, as a physiological process, during the embryonic development of multicellular organisms. In the retina, which belongs to the central nervous system, at least two phases of cell death have been reported to occur during development. An early phase takes place concomitant with the processes of neurogenesis, cell(More)
—Ecosystem responses to interannual weather variability are large and superimposed over any long-term directional climatic responses making it difficult to assign causal relationships to vegetation change. Better understanding of ecosystem responses to interannual climatic variability is crucial to predicting long-term functioning and stability.(More)
PURPOSE To examine the effects of glia-derived and brain-derived neurotrophic factors on survival and morphology of cultured retinal ganglion cells (RGCs) from adult porcine retina. METHODS Adult porcine retinas were dissociated and cultured in different conditions: (1) on laminin- and poly-D-lysine-coated coverslips in chemically defined medium (CDM);(More)
This work aimed to evaluate the potential role of the 5-HT(7) receptor in nociception secondary to a sensitizing stimulus in mice. For this purpose, the effects of relevant ligands (5-HT(7) receptor agonists: AS-19, MSD-5a, E-55888; 5-HT(7) receptor antagonists: SB-258719, SB-269970; 5-HT(1A) receptor agonist: F-13640; 5-HT(1A) receptor antagonist:(More)
We validated the pig eye as a model of glaucoma, based on chronic elevation of intraocular pressure (IOP). IOP was elevated by cauterising three episcleral veins in each of the left eyes of five adult pigs. Right eyes were used as controls. Measurement of IOP was performed during the experiment with an applanation tonometer (Tono-Pen). Five months after(More)
Glial cells are thought to protect neurons from various neurological insults. When there is injury to retina, Müller cells, which are the predominant glial element in the retina, undergo significant morphological, cellular and molecular changes. Some of these changes reflect Müller cell involvement in protecting the retina from further damage. Müller cells(More)
PURPOSE To characterize and compare the expression of neurotrophins (NTs) and their receptors within adult porcine retinal ganglion cells (RGCs) in vivo and in vitro. METHODS The distribution of nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3) and -4 (NT-4), and their high-affinity receptors TrkA, TrkB, TrkC and(More)
PURPOSE Neurofilaments (NF) are neuronal cytoskeletal components and immunostaining against them has been used to visualize retinal ganglion cells (RGC) and their axons. Since the RGC cytoskeleton exhibits differential damage in diseases such as glaucoma, we examined the distribution of light, medium, and heavy NF subunits (NF-L, NF-M, and NF-H(More)
Teleost retinas adapted to light show numerous spinules invaginated in the cone pedicles and small nematosomes in the distal horizontal cells. Darkness induces the dissolution of spinules and the presence of large and numerous nematosomes. The aim of this work is to study the influence of optic nerve integrity on spinule formation/dissolution and changes in(More)
Brain-derived neurotrophic factor (BDNF) has been implicated in stimulating retinal ganglion cell (RGC) survival and axonal regeneration in rodent animal models in vivo and in vitro, but very little data are available on neurotrophin effects in higher mammals. We hence analysed BDNF signalling in primary cultures of adult pig RGC. As detected by(More)