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We endeavored to develop a method for viability determination of solventogenic clostridia and to apply it for monitoring acetone–butanol–ethanol (ABE) fermentation. Six fluorescent probes (propidium iodide [PI], ethidium bromide, fluorescein diacetate, carboxyfluorescein diacetate [cFDA], rhodamine 123, bis-(1,3-dibutylbarbituric acid)trimethine oxonol(More)
The total yield of ergosterol produced by the fermentation of the yeast Saccharomyces cerevisiae depends on the final amount of yeast biomass and the ergosterol content in the cells. At the same time ergosterol purity-defined as percentage of ergosterol in the total sterols in the yeast-is equally important for efficient downstream processing. This study(More)
The effect of lignocellulosic hydrolysate of crushed corn cobs on the kinetics of growth and lactic acid production of Lactobacillus casei and L. lactis in the cell retention continuous culture was studied. The continuous cultivations were carried out in a continuous flow stirred bioreactor combined in a recycle loop with an ultrafiltration module retaining(More)
Clostridium pasteurianum forms acetic and butyric acids in an initial growth phase, which is a typical feature of clostridial acetone-butanol fermentation where an initial accumulation of acids is followed by production of solvents 1-butanol, acetone and ethanol. The initiation of the solvent production coupled with endospore formation leads to decrease of(More)
This review provides current information on the production of ethanol from lignocellulosic biomass, with the main focus on relationships between process design and efficiency, expressed as ethanol concentration, yield and productivity. In spite of unquestionable advantages of lignocellulosic biomass as a feedstock for ethanol production (availability,(More)
This review emphasises the fact that studies of acetone-butanol-ethanol (ABE) fermentation by solventogenic clostridia cannot be limited to research on the strain Clostridium acetobutylicum ATCC 824. Various 1-butanol producing species of the genus Clostridium, which differ in their patterns of product formation and abilities to ferment particular(More)
A series of continuous fermentations were carried out with a production strain of the yeast Saccharomyces cerevisiae in a membrane bioreactor. A membrane separation module composed of ultrafiltration tubular membranes retained all biomass in a fermentation zone of the bioreactor and allowed continuous removal of fermentation products into a cell-free(More)
For automated process control it is necessary to know the data of the actual substrate concentration, activity and concentration of the biomass at any time of the fermentation. We have therefore proposed models balancing either the main biogenic elements or the key element only, which fulfil these demands. By measuring CO2 production rate and O2 uptake rate(More)
The effect of lignocellulosic hydrolysate of crushed corn mobs on the growth and lactic acid formation in a continuous culture ofLactobacillus casei andL. lactis at dilution rate 0.08–0.3/h was studied. A simple physiological model of the process was derived from computer-aided analysis of the data which relates bacterial growth, lactic acid formation to(More)
Physiological changes in populations of Clostridium beijerinckii and Clostridium tetanomorphum were monitored by fluorescence staining and flow cytometry. To estimate the number of metabolically active cells in exponential growth, a combination of the dyes propidium iodide and carboxy fluorescein diacetate appeared to be a good choice for both species.(More)