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3-Hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase, a key regulatory enzyme involved in cholesterol biosynthesis, has recently been reported to be present in rat liver peroxisomes (Keller, G.A., M.C. Barton, D.J. Shapiro, and S.J. Singer, 1985, Proc. Natl. Acad. Sci. USA, 82:770-774). Immunoelectron labeling of ultrathin frozen sections of normal(More)
The gene coding for the Neurospora crassa metallothionein protein was chemically synthesized and cloned into the fusion expression vectors pMal-c and pMal-p. Cell-fractionation experiments demonstrated the proper localization of the pMal-c- and pMal-p- expressed proteins to the cytosol and periplasm of the bacteria respectively. Control bacteria as well as(More)
A recombinant Escherichia coli expressing the Neurospora crassa metallothionein gene (NCP) has previously been shown to remove low levels of Cd and other metals from solution. For further development as a biosorbent, the encapsulation of the NCP is investigated by various matrices. The NCP was encapsulated in alginate, chitosan-alginate or(More)
A gene coding for a de novo peptide sequence containing a metal binding motif was chemically synthesized and expressed in Escherichia coli as a fusion with the maltose binding protein. Bacterial cells expressing the metal binding peptide fusion demonstrated enhanced binding of Cd2+ and Hg2+ compared to bacterial cells lacking the metal binding peptide. The(More)
To evaluate the effect of synthetic DNA oligomers on regulation of bacterial genes in vivo, we tested 63 oligomers of variable length and chemistry for their ability to selectively suppress light production in the bioluminescent marine organism, Vibrio fischeri. Phosphodiester, phosphorothioate, and mixed backbone oligomers were designed to be lux gene(More)
  • M Pazirandeh
  • 1996
The potential utility of a recombinant E. coli expressing the Neurospora crassa metallothionein gene (NCP) as a heavy metal biosorbent was investigated. It was shown that the NCP was capable of efficiently removing low levels of several metals (including cadmium, lead, and mercury) from solutions. The reusability of the NCP was demonstrated through 5 cycles(More)
The bacterium Vibrio parahaemolyticus was tested for its ability to internalize unmodified as well as modified DNA oligomers without attempting to permeabilize the cells. These experiments were conducted to establish whether it may be feasible to employ antisense oligomers for control of gene expression in Vibrio species without heat-shocking or(More)
The performance modeling of computer systems plays an important role in the system engineering life cycle. Historically, however, this important role has not been recognized, and often it has been treated as an obligation. The reason for this diminished stature has many causes, but foremost is that performance modeling has failed to position itself as an(More)
Let S(g) be the symmetric algebra over the complexifica-tion 9 of the real semisimple Lie algebra g. For u £ S(g), d(u) is the corresponding differential operator on g. 3)(g) denotes the algebra generated by d(S(g)) and multiplication by polynomials on g. For any open set U C t, Diff (LO is the algebra of differential operators with C°°-coefficients on U.(More)
Rhapidosomes are tubular microstructures composed of proteins that are found in a variety of bacteria and algae. These structures, which are resistant to disruption by many denaturing agents, have potential application as a biomaterial and may serve as a new model for the study of self assembly. When rhapidosomes were purified and analysed by SDS-PAGE the(More)
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