Mohieddine Missaoui

Learn More
MOTIVATION Microbial diversity is still largely unknown in most environments, such as soils. In order to get access to this microbial 'black-box', the development of powerful tools such as microarrays are necessary. However, the reliability of this approach relies on probe efficiency, in particular sensitivity, specificity and explorative power, in order to(More)
MOTIVATION The use of DNA microarrays allows the monitoring of the extreme microbial diversity encountered in complex samples like environmental ones as well as that of their functional capacities. However, no probe design software currently available is adapted to easily design efficient and explorative probes for functional gene arrays. RESULTS We(More)
The microbial world represents the most important and diverse group of organisms living on earth. Because of this huge microbial bio complexity, high-throughput molecular tools allowing simultaneous analysis of existing populations are well adapted. Oligonucleotide micro array technologies have been widely used for gene detection and gene expression(More)
In the context of new metabolic pathways discovery, a full backtranslation of oligopeptides can be a promising approach. When studying complex environments where the composing microorganisms are unknown it is also preferable to have all the complete nucleic sequences corresponding to an enzyme of interest. In this paper, we revisit the existing(More)
DNA hybridization methods have become the most widely used tools in molecular biology to identify organisms and evaluate gene expression levels. PCR (Polymerase Chain Reaction)-based methods, fluorescent in situ hybridization (FISH) and the recent development of DNA microarrays as a high throughput technology need efficient primers or probes design.(More)
Phylogenetic Oligonucleotide Arrays (POAs) were recently adapted for studying the huge microbial communities in a flexible and easy-to-use way. POA coupled with the use of explorative probes to detect the unknown part is now one of the most powerful approaches for a better understanding of microbial community functioning. However, the selection of probes(More)
In order to design microarray oligonucleotides, in the context of new metabolic pathways discovery, it appears that a full backtranslation of oligopeptides is a promising approach. Protein to DNA reverse translation is a time-consuming task that can provide unreasonable quantities of data. This is why most current applications use genetic degenerated code(More)
  • 1