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Phage display technologies were used to produce single-chain antibodies (scFv) against breast cancer cell line (MCF-7). The mouse B cell hybridoma line C3A8 was the starting material, which generates a monoclonal antibody against breast cancer cells (MCF-7). The best candidate scFv sequences, based on (ELISA) screening data were sub-cloned into HB2151 host… (More)