Mobeen Ahmed Khan

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During 2006-2011, 331 red chilli samples (226 whole, 69 powdered and 36 crushed) were collected from all over Pakistan for the estimation of total aflatoxins (AFs = AFB1 + AFB2 + AFG1 + AFG2) contamination by thin layer chromatography (TLC). Mean AFs levels in whole, powdered and crushed chillies were 11.7, 27.8 and 31.2 µg kg(-1), respectively. AFs levels(More)
Advancement in the field of analytical food-chemistry has explored various experimental techniques for aflatoxins (AFs) quantification. The present study was aimed to compare four different techniques; thin-layer chromatography (TLC), high-performance liquid chromatography (HPLC), liquid chromatography-tandem mass spectrometry (LC-MS/MS) and enzyme-linked(More)
BACKGROUND The objective of this study was to determine the distribution of an economically-important class of mycotoxins, the aflatoxins (AFs) in Pakistani Brown Rice. METHODS A total of 262 of brown rice samples were collected from different vendors during July 2006 to June 2011. Samples were analyzed for the occurrence of aflatoxin B1 (AFB1), B2(More)
A novel, reliable and rapid high-performance liquid chromatography (HPLC) method with post-column derivatization was developed and validated. The HPLC method was used for the simultaneous determination of aflatoxin B1 (AFB1), B2 (AFB2), G1 (AFG1) and G2 (AFG2) in various cereals and grains. Samples were extracted with 80:20 (v/v) methanol:water and purified(More)
Aflatoxin B1 (AFB1) levels were evaluated in betel nuts (Areca catechu L.) being imported to Pakistan during 2010-2011. In total, 278 betel nut samples (India = 21, Indonesia = 51, Sri-Lanka = 34 and Thailand = 172) were received from the Department of Customs and were analysed by thin layer chromatography (TLC). All Indian origin betel nuts showed AFB1(More)
The sputa of 231 cases, who were admitted to Ojha Institute of Chest Diseases, Karachi were studied in order to compare the efficiency and reliability of three pretreatment methods viz. Standard method, Marks' method (1959), and Becker's method (1961). Out of the 231 specimens, 132 were film positive while 99 were film negative. 141 cases were found(More)
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