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The complete nucleotide sequence of the tobacco retrotransposon Tto1, one of the few active retrotransposons of plants, was determined. The sequence analysis suggests that Tto1 carries all functions required for autonomous transposition through reverse transcription. Gene organization and the nature of the transcription product suggest that Tto1 uses a gene(More)
Two enzymes from soybean (Glycine max L. Merr. cv Harosoy 63) cotyledons released elicitor-active carbohydrates from cell walls of the phytopathogenic fungus Phytophthora megasperma f.sp. glycinea. They were identified as isoenzymes of beta-1,3-endoglucanase (EC 3.2.1.39) with isoelectric points of pH 8.7 and 10.5. The pI 10.5 enzyme was extracted in the(More)
Soybean membrane preparations specifically bound [(14)C]mycolaminaran, a branched beta-1,3-glucan produced by Phytophthora sp. which elicits production of the phytoalexin glyceollin in soybean tissues. A Scatchard plot of the binding data disclosed the presence of a single affinity class of binding sites with a K(d) value of 11.5 micromolar for the glucan.(More)
A soluble elicitor of glyceollin accumulation was released from insoluble mycelial walls of Phytophthora megasperma var. sojae after incubation with soybean cotyledon tissue for as little as 2 minutes. Various enzymic and chemical treatments of the released elicitor indicated that the activity resided in a carbohydrate moiety, and gel filtration disclosed(More)
Three unique classes of carbohydrates were isolated from the hyphal cell walls of Phytophthora megasperma f.sp. glycinea (Pmg) and compared with other substances for their activity as elicitors of the phytoalexin glyceollin in soybean tissues. Glucomannans extracted from cell walls with soybean beta-1,3-endoglucanase were purified and proved to be the most(More)
The pterocarpinoid phytoalexins, glyceollin, pisatin, and phaseollin, stimulated adventitious root formation in a mung bean rooting bioassay only in the presence of indoleacetic acid (rooting cofactor activity). Relatively low (5 to 50 micrograms per milliliter) concentrations of the phytoalexins were effective. The phytoalexins also increased the numbers(More)
Actinomycin D inhibited the synthesis of poly(A)-containing messenger RNA in healthy soybean (Glycine max [L.] Merr. cv. Harosoy 63) hypocotyls and in hypocotyls inoculated with the pathogenic fungus Phytophthora megasperma var. sojae A. A. Hildb., but had little effect on protein synthesis within 6 hours. Blasticidin S, conversely, inhibited protein(More)
(1(OH),2,4,5/1,3)-5-amino-1-C-(hydroxymethyl)-1,2,3,4-cyclohexalletetrol. Valiolamine has more potentα-glucosidase inhibltory activity against porcine intestinal sucrase, maltase and isomaltase than valienamine, vahdamine and hydroxyvalidamine which were reported as building blocks of validamycins and microbial oligosaccharldeα 一glucosidase inhibitors. In(More)
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