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1. The aim of the present work was to study the role of Na+, Ca2+ and cyclic nucleotides in stimulus-secretion coupling in rat pancreatic acinar cells utilizing Ca2+ specific ionophore A23187 as well as ionophores X-537A and BrX-537A which transport both monovalent and divalent cations across biological membranes. 2. X-537A, BrX-537A and A23187 increased(More)
1. The effect of octapeptide of cholecystokinin-pancreozymin (CCK(8)), bethanechol, cholera toxin, glucagon and vasoactive intestinal polypeptide (VIP) on amylase secretion and lactic dehydrogenase (LDH) release from isolated rat pancreatic acini was studied.2. In isolated rat pancreatic acini, in the absence of theophylline in the medium, amylase secretion(More)
1. The effect of membrane stabilizers and cytochalasin-B on amylase secretion, basal and induced by ionophore A23187, CCK-PZ, bethanechol and glucagon, was studied in dissociated mouse pancreatic acinar cells. 2. Cytochalasin-B did not affect basal or secretagogue-stimulated amylase secretion. 3. Membrane stabilizers [thymol (10(-7)-10(-4) M),(More)
The effect of tumor necrosis factor alpha (TNF alpha) on lymphocyte beta 2-adrenergic receptor response is reported. Isoproterenol-mediated cyclic-3,5-adenosine monophosphate (cAMP) production by intact lymphocytes preincubated with isotonic buffer or TNF alpha was studied. TNF alpha at 1 ng/ml and 100 ng/ml reduced isoproterenol-stimulated cAMP generation(More)
1. Interaction of Ca2+ and cyclic nucleotides in stimulus-secretion coupling in rat pancreas in vitro was studied utilizing the divalent cation inophore A23187. phosphodiesterase inhibitors, cyclic nucleotides and cholera toxin. 2. Amylase secretion was increased by the ionophore in the presence of extracellular Ca2+ in a dose-dependent fashion. Activation(More)
Isolation of pure acinar cells of the rat pancreas was achieved employing counterflow sedimentation filtration technique (CSFT). The preparation of purified acinar cells contained an occasional red blood cell (RBC, 200:1) with total absence of endocrine and duct cells. A significant stimulation of amylase secretion from isolated pure acinar cells was(More)
1. Effect of glucagon on amylase secretion and lactic dehydrogenase (LDH) release from functionally intact dissociated pancreatic acinar cells and acini was studied. 2. In dissociated rat pancreatic acinar cells, the rate of amylase secretion was increased by 70% with bethanechol (maximally effective concentration, 10(-4) M) and 125% with A23187 (10(-5) M),(More)