Miyuki Mekuchi

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We examined morphological changes and molecular mechanisms of ion regulation in mitochondrion-rich (MR) cells of Japanese eel acclimated to different environmental salinities. Electron microscopic observations revealed that the apical membrane of MR cells appeared as a flat or slightly projecting disk with a mesh-like structure on its surface in eel(More)
Marine teleosts produce Ca precipitates in the intestine as a product of osmoregulation. Ca precipitates are formed by a chemical reaction of Mg(2+) and Ca(2+) derived from ingested seawater with bicarbonate (HCO(3)(-)). It has been reported that HCO(3)(-) originates from the intestine; however, the pancreas is predicted to be another organ that may supply(More)
In the present study, we aimed to elucidate the mechanisms of intestinal Na(+) and Cl(-) absorption in Japanese eel, focusing on electroneutral cation-Cl(-) cotransporters, NKCC2β and NCCβ, expressed in the intestinal tract. First, we cloned cDNAs encoding NKCC2β and NCCβ from the intestinal tract of Japanese eel. In both freshwater- and seawater-acclimated(More)
Ovarian low density lipoproteins (LDL) such as vitellogenin (Vg) are the precursors of the major yolk protein vitellin, and constitute the major source of nutrients serving the developing embryo. The objective of this study was to gain a better understanding of crustacean egg development by focusing on the process of Vg internalization by its receptor(More)
Marine teleosts are known to produce white feces, which is often referred to as Ca precipitates. Ca precipitates have been suggested to be a product of osmoregulation. In the present study, we examined the physicochemical nature of Ca precipitates, and possible involvement of Ca precipitate formation in hyposmoregulatory processes in seawater-acclimated(More)
Reabsorption of monovalent ions in the kidney is essential for adaptation to freshwater and seawater in teleosts. To assess a possible role of Na(+)/H(+) exchanger 3 (NHE3) in renal osmoregulation, we first identified a partial sequence of cDNA encoding NHE3 from the Japanese eel kidney. For comparison, we also identified cDNAs encoding kidney specific(More)
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