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Six monoclonal antibodies, three each of human IgG1 and IgG2 subclasses, were obtained from human-mouse hybridomas. Structural study of their asparagine-linked sugar chains was performed to elucidate the regulatory mechanism of secreted monoclonal IgG glycosylation. The sugar moieties were quantitatively released as oligosaccharides from the polypeptide(More)
The effect of the atrial natriuretic factor (ANF) on K+ channel opener-induced glibenclamide-sensitive K+ currents was studied using follicle-enclosed Xenopus oocytes. K+ currents induced by the K+ channel opener Y-26763 were potentiated by ANF (0.5-50 nM) in a concentration-dependent manner. 50 nM ANF increased the peak amplitude of the current by 59.4 +/-(More)
We isolated a temperature-sensitive mutant from mouse FM3A cells, designated as tsFT20, the DNA polymerase alpha activity of which is heat-labile. A hybrid clone (M6-39 cells) between human cells and tsFT20 cells contained one or two human chromosomes. M6-39 cells (primary hybrid) were exposed to gamma-ray and re-fused with tsFT20, after which we isolated(More)
An interspecific cell hybrid was constructed between a temperature-sensitive mutant cell line of mouse FM3A cells (FT20-M6, a 6-thioguanine-resistant derivative of tsFT20) that has heat-labile DNA polymerase alpha and a human diploid fibroblast derived from a patient with the fragile X syndrome. After extensive segregation of the human chromosomes, a hybrid(More)
−This paper describes a new redundant fault identification algorithm with Exclusive-OR circuit reduction. The experimental results using this algorithm with a FAN-based test pattern generation algorithm show nearly 100% fault coverage for complex arithmetic logic circuits. Moreover we achieved 99.99% fault coverage applying this algorithm with a weighted(More)
The chromosomal location of human DNA polymerase alpha gene was determined by studies on somatic cell hybrids between a temperature-sensitive mutant cell line of mouse FM3A cells and normal human lymphocytes or a line of human diploid fibroblasts derived from a patient with the fragile X syndrome. A temperature-sensitive mutant, FT20-M6, a(More)
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