Mitsuru Harada

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RNA interference (RNAi) is one of the most promising new approaches for disease therapy. The design of a dumbbell-shaped nanocircular RNA allows it to act as a short interfering RNA (siRNA) precursor. To optimize the design, we studied the relationship between the nanostructure and RNAi activity by synthesizing various RNA dumbbells. An RNA dumbbell with a(More)
This paper describes circuit techniques for a 1-V operation Bluetooth PF transceiver in 0.2-/spl mu/m CMOS/SOI. Folded LC-tuned and folded transistor-current-source circuits achieve 1-V operation of the RF transceiver. Tuning circuits and a bias generation circuit for robust transceiver operation are explained.
We demonstrate a generation of tandem repeats of a malachite green (MG) RNA aptamer using rolling circle transcription. To keep the higher-order structure of each aptamer on long RNA, we designed a sequence of circular DNA with a 14-base linker. T7 RNA polymerase was superior to Escherichia coli RNA polymerase in the specific transcription of the MG RNA(More)
We designed and synthesized dumbbell-shaped nanocircular RNAs for RNA interference applications, which consist of a stem and two loops(1). RNA dumbbells are specifically recognized and cleaved by the human Dicer enzyme, and are thus transformed into double-stranded RNA in cells, although this RNA is resistant to degradation in serum. The structure was(More)
For adaptive reduction of power consumed by wireless receivers, we developed the first intra-symbol intermittent (ISI) RF front-end with 0.35-µm CMOS technology. In the demodulation mechanism, the RF output of the LNA is down-converted to an IF by the mixer, and the LNA and mixer operate synchronously and intermittently within a single symbol-length.(More)
A tetrathionate-decomposing enzyme that catalyzes the decomposition of tetrathionate into thiosulfate and sulfate was purified to homogeneity from tetrathionate-grown 1:hiobacitVbes thiooxiduns. The enzyme had an apparent molecular weight of 104,OOO, and was composed of two identica] subunits {MW=58,ooO) as estimated by sodi"m dodecyl su]fate-polyacrylamide(More)