Misumi Kataoka

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An NADPH-dependent aldehyde reductase (ALR) isolated from a red yeast, Sporobolomyces salmonicolor, catalyzes the reduction of a variety of carbonyl compounds. To investigate its primary structure, we cloned and sequenced the cDNA coding for ALR. The aldehyde reductase gene (ALR) comprises 969 bp and encodes a polypeptide of 35,232 Da. The deduced amino(More)
BACKGROUND Enzymatic removal of hemicellulose components such as xylan is an important factor for maintaining high glucose conversion from lignocelluloses subjected to low-severity pretreatment. Supplementation of xylanase in the cellulase mixture enhances glucose release from pretreated lignocellulose. Filamentous fungi produce multiple xylanases in their(More)
The photoproduction of prompt photons, together with an accompanying jet, has been studied in ep collisions at a centre-of-mass energy of 318 GeV with the ZEUS detector at HERA using an integrated luminosity of 77 pb −1. Cross sections were measured for the transverse energy of the photon and the jet larger than 5 and 6 GeV, respectively. The differential(More)
The dependence of dijet production on the virtuality of the exchanged photon, Q 2 , has been studied by measuring dijet cross sections in the range 0 < ∼ Q 2 < 2000 GeV 2 with the ZEUS detector at HERA using an integrated luminosity of 38.6 pb −1. Dijet cross sections were measured for jets with transverse energy E jet T > 7.5 and 6.5 GeV and(More)
Talaromyces cellulolyticus (formerly known as Acremonium cellulolyticus) is one of the mesophilic fungi that can produce high levels of cellulose-related enzymes and are expected to be used for the degradation of polysaccharide biomass. In silico analysis of the genome sequence of T. cellulolyticus detected seven open reading frames (ORFs) showing homology(More)
The ZEUS detector has been used to study dissociation of virtual photons in events with a leading proton, γ ⋆ p → Xp, in e + p collisions at HERA. The data cover photon virtualities in two ranges, 0.03 < Q 2 < 0.60 GeV 2 and 2 < Q 2 < 100 GeV 2 , with M X > 1.5 GeV, where M X is the mass of the hadronic final state, X. Events were required to have a leading(More)
O-Phosphoserine sulfhydrylase (OPSS) synthesizes cysteine from O-phospho-l-serine (OPS) and sulfide. We have determined the three-dimensional structures of OPSS from hyperthermophilic archaeon Aeropyrum pernix K1 (ApOPSS) in complex with aminoacrylate intermediate (AA) formed from pyridoxal 5′-phosphate with OPS or in complex with cysteine and compared them(More)
The hyperthermophilic glycoside hydrolase family endocellulase 12 from the archaeon Pyrococcus furiosus (EGPf; Gene ID PF0854; EC 3.2.1.4) catalyzes the hydrolytic cleavage of the β-1,4-glucosidic linkage in β-glucan in lignocellulose biomass. A crystal of EGPf was previously prepared at pH 9.0 and its structure was determined at an atomic resolution of(More)
β-Glucosidase from Pyrococcus furiosus (BGLPf) is a hyperthermophilic tetrameric enzyme which can degrade cellooligosaccharides to glucose under hyperthermophilic conditions and thus holds promise for the saccharification of lignocellulosic biomass at high temperature. Prior to the production of large amounts of this enzyme, detailed information regarding(More)
One of the β-glucosidases from Pyrococcus furiosus (BGLPf) is found to be a hyperthermophilic tetrameric enzyme that can degrade cellooligosaccharides. Recently, the crystal structures of the tetrameric and dimeric forms were solved. Here, a new monomeric form of BGLPf was constructed by removing the C-terminal region of the enzyme and its crystal structure(More)