Mirjam S. van de Vliet

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We explored the relationships between perturbation-driven population decline and genetic/genotypic structure in the clonal seagrass Posidonia oceanica, subject to intensive meadow regression around four Mediterranean fish-farms, using seven specific microsatellites. Two meadows were randomly sampled (40 shoots) within 1,600 m2 at each site: the “impacted”(More)
A microsatellite library was developed using genomic DNA of the Parsley frog, Pelodytes punctatus, an amphibian species which inhabits Mediterranean temporary pond systems. Number of alleles and heterozygosity ranged from 10 to 25 and from 0.66 to 0.90, respectively. Cross-species amplification was successful for 13 of the 15 developed loci for the related(More)
Nine highly polymorphic microsatellite markers were isolated and characterized for the Western Spadefoot, Pelobates cultripes. Remarkably, for this amphibian species high numbers of microsatellites were found as part of larger repeat containing regions, making primer design difficult. For nine loci, primers were designed successfully and genotyping of(More)
Species of the family Syngnathidae are increasingly being investigated for conservation purposes but also for mating system and genetic parentage studies. The availability of highly polymorphic markers will be of great importance to conduct these kinds of studies. This paper describes the development and characterization of 10 polymorphic microsatellite(More)
For population genetic analyses of Parsley frogs in Iberia we initially used microsatellite markers previously developed for Pelodytes punctatus in southwest Portugal. However, several loci indicated a strong signal of amplification failure for individuals from northern Spain. Cryptic species or genetic entities are suspected to occur in southwest Portugal(More)
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