Miquel D. Antoine

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An improved data analysis method is described for rapid identification of intact microorganisms from MALDI-TOF-MS data. The method makes no use of mass spectral fingerprints. Instead, a microorganism database is automatically generated that contains biomarker masses derived from ribosomal protein sequences and a model of N-terminal Met loss. We(More)
JOHNS HOPKIN The best defensive measures against a biological weapons attack are to determine the agent(s) used and rapidly identify the exposed populations. However, technologies to accomplish these goals are only in their infancy. Through Defense Advanced Research Projects Agency sponsorship, the APL Tiny Time-of-Flight (TOF) Mass Spectrometer Program is(More)
The design and operation of an arrayed time-of-flight (TOF) mass spectrometer for simultaneous data acquisition from multiple samples is described. Versions of the instrument employ sets of two or four linear or reflectron mass analyzers. They are housed in the same vacuum chamber and utilize the same laser for ion desorption. Instrument performance is(More)
The intron-containing gene encoding human ribosomal protein S6 (rpS6), the major phosphoprotein in the mammalian ribosome, has been cloned. Using a PCR based cloning strategy we have isolated the rpS6 intron-containing gene in the presence of its many processed pseudogenes and determined the DNA sequence of the entire gene and its upstream and downstream(More)
miniaturized MALDI-TOF mass spectrometer has been in development at APL for the last several years. Besides meeting the mechanical design challenges in taking a bench-top–sized instrument and shrinking it down for easy and rapid deployment with autonomous operation, sample collection and preparation methods have been developed to analyze, detect, and(More)
A rapid method to determine drug resistance in bacteria based on mass spectrometry is presented. In it, a mass spectrum of an intact microorganism grown in drug-containing stable isotope-labeled media is compared with a mass spectrum of the intact microorganism grown in non-labeled media without the drug present. Drug resistance is determined by predicting(More)
The capability to rapidly and confidently determine or confirm the sequences of short oligonucleotides, including native and chemically-modified DNA and RNA, is important for a number of fields. While matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometry (MS) has been used previously to sequence short oligonucleotides,(More)
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