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We monitored developmental alterations in the morphology of dendritic spines in primary cultures of hippocampal neurons using confocal laser scanning microscopy (CLSM) and the fluorescent marker Dil. Dissociated rat hippocampal neurons were plated on polylysine-coated glass cover slips and grown in culture for 1-4 weeks. Fixed cultures were stained with Dil(More)
Methylphenidate (MPH) administration to adolescent rodents produces persistent region-specific changes in brain reward circuits and alterations of reward-based behavior. We show that these modifications include a marked increment of serotonin (5-hydroxy-tryptamine) receptor type 7 (Htr7) expression and synaptic contacts, mainly in the nucleus accumbens, and(More)
Rat hippocampal neurons, grown in dissociated culture for about 18 days, were exposed for 6 h to three days to stimuli which cause either an increase (GABAA antagonists, bicuculline or picrotoxin), or decrease (tetrodotoxin) in spontaneous neuronal activity. Individual neurons were stained with 1,1'-dioctadecyl-3,3,3',3'-tetramethyl-indocarbocyanine(More)
BACKGROUND Many brain areas participate to supraspinal control of nociception. In these regions, few studies have investigated the role of glial cells in supraspinal plasticity and the effect of 7-day intrathecal nerve growth factor-like (BB14®, Blueprint Biotech, Milano, Italy) treatment. METHODS In male Sprague-Dawley rats, we evaluated by(More)
Mitochondrial metabolism impairment has been implicated in the pathogenesis of several neurodegenerative disorders. In the present work, we combined electrophysiological recordings and microfluorometric measurements from cholinergic interneurons obtained from a rat neostriatal slice preparation. Acute application of the mitochondrial complex I inhibitor(More)
Studies were carried out in the rat to determine if the area postrema (AP), a medullary circumventricular organ, might be involved in the control of vasopressin (VP) release. The data from this study demonstrate the existence of direct neural connections between the AP and the hypothalamic VPergic neurons of the supraoptic nucleus (SON) as showed by the(More)
In this work, we illustrate the basic development of the constrained molecular dynamics applied to the N-body problem in nuclear physics. The heavy computational tasks related to quantum effects, to the Fermionic nature of the system have been resolved out by defining a set of transformations based on the concept of impulsive forces. In particular, in the(More)
  • Concettina Ssa, Tutore Enrico Guerra, Pagello, La Tesi, Alla Mia Mamma E Al, Mio Papà +5 others
  • 2002
This dissertation describes the work done by the author in the period of his Ph.D. in the field of omnidirectional vision. After a general presentation on omnidirectional vision, the dissertation focuses on omnidirectional vision for mobile robotics. Each chapter of the dis-sertation covers a different topic in omnidirectional vision for mobile robotics.(More)
The intraseptal administration of the neurotoxin kainic acid (KA) induced a significant depletion of the neuronal population and glial proliferation in medial septal areas. The behavioral effects induced by this selective destruction of the neurons indigenous to the medial septal areas were investigated. KA produced a marked increase in urine output and a(More)
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