Minoru Otokawa

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Action of gamma-aminobutyric acid (GABA) in the preoptic area and anterior hypothalamus (PO/AH) has been implicated to regulate body temperature (T(b)). However, its precise role in thermoregulation remains unclear. Moreover, little is known about its release pattern in the PO/AH during active thermoregulation. Using microdialysis and telemetry techniques,(More)
A microbore column high-performance liquid chromatography (HPLC) system was used to measure neurotransmitters in tissue extracts and in vivo microdialysates obtained from the preoptic area (PO) and anterior hypothalamus (AH) of rats. The extracts contained norepinephrine, epinephrine, 3,4-dihydroxyphenylacetic acid (DOPAC), dopamine, 5-hydroxyindoleacetic(More)
To examine the role of monoamines and amino acids in thermoregulation, we measured their concentrations in the preoptic area and anterior hypothalamus (PO/AH) in exercising rats, using an in vivo microdialysis technique. Body temperature (Tb) was monitored using a telemetry system. Tb increase by about 1.0 degrees C in the first 15 min of treadmill exercise(More)
We investigated the effects of environmental light and darkness on thermoregulation during both daytime and nighttime by monitoring body temperature (T(b)) and physical activity of rats using a telemetry system. Experiments were performed in both resting and exercising rats. In resting rats, lights-off during the daytime resulted in an increase in both T(b)(More)
We recently reported that perfusion of tetrodotoxin (TTX) into the preoptic area and anterior hypothalamus (PO/AH), by using a microdialysis technique, induced an increase in body temperature (Tb) under normal and hot ambient temperatures (23 and 35 degrees C) in freely moving rats. However, the procedure had no effect on Tb under a cold ambient temperature(More)
Although many studies has been shown that serotonin (5-HT) in the preoptic area and anterior hypothalamus (PO/AH) is important for regulating body temperature (Tb), the exact role is not established yet due to conflicting results probably related to experimental techniques or conditions such as the use of anesthesia. The purpose of present study was to(More)
To clarify the role of the preoptic area and anterior hypothalamus (PO/AH) on thermoregulatory system and the effects of serotonergic innervation from the median raphe nucleus (MRN) on body temperature (Tb), we perfused tetrodotoxin (TTX) solution into the PO/AH or MRN by using a microdialysis technique at different ambient temperatures (5, 23 and 35(More)
The constituent elements of the gills of Aplysia kurodai and A. juliana were examined for the presence of biogenic amines using histochemical, immunocytochemical, and HPLC techniques. Aminergic elements were revealed by glyoxylic acid-induced fluorescence in the branchial nerve, branchial ganglion, branchial vessels, and pinnules in both species. Three(More)
In crustacean neurogenic hearts, the myocardium contracts under the tight control of rhythmically active neurons of the cardiac ganglion located inside the heart. We demonstrated that the myocardium of Bathynomus doederleini was sensitive to glutamate, quisqualate, and kainate, and that the tension of myocardial cells developed in a dose-dependent manner.(More)