Minoru Makita

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Recent studies have revealed the presence of intracellular lipid droplets in wide variety of species. In mammalian cells, there exist proteins specifically localize in lipid droplets. However, the protein profile in the droplet remains yet to be clarified. In this study, a fraction enriched with lipid droplets was isolated from a human hepatocyte cell line(More)
Lipid droplets (LDs) function as intracellular storage depots of neutral lipids. Recently, we identified long-chain acyl-coenzyme A synthetase 3 (ACSL3) as a major LD-associated protein in the human hepatocyte cell line HuH7. In this study, we investigated whether droplet-associated ACSL is involved in lipid metabolism in LDs. Addition of oleic acid (OA) to(More)
A capillary gas chromatographic (GC) method for the simultaneous determination of urinary hippuric acid (HA) and o-, m- and p-methylhippuric acids (MHAs), metabolites of toluene and o-, m- and p-xylenes, respectively, is described. These metabolites are converted into their isopropyl derivatives by extractive alkylation with tetrahexylammonium ion as(More)
We examined the hypothesis that different types of ingested matter may be processed at different rates by a marine microzooplankter. Ciliates were exposed to a prey item and then diluted at 1: 100. Disappearance or digestion of inert (fluorescent microspheres) and digestible prey items (heatkilled and fluorescently labeled Synechococcus, natural(More)
A selective and sensitive gas chromatographic method for the analysis of lipoic acid in biological samples has been developed. After base hydrolysis of the sample, the liberated lipoic acid was converted into its S,S-diethoxycarbonyl methyl ester derivative and measured by gas chromatography using a DB-210 capillary column and a flame photometric detector.(More)
A selective and sensitive method has been developed for the analysis of free and bound forms of O-phosphoamino acids, such as O-phosphoserine, O-phosphothreonine and O-phosphotyrosine, in urine samples by gas chromatography (GC). For free O-phosphoamino acid analysis, the urine sample was extracted with trichloroacetic acid and run through an ion exchange(More)