Mingxin Fan

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We have previously demonstrated that cyclothiazide (CTZ) is a potent convulsant drug inducing robust epileptiform activity in hippocampal neurons both in vitro and in vivo. Here we further establish an animal model for CTZ-induced behavioral seizures in freely moving rats. Microinjection of CTZ into the left ventricle dose-dependently induced robust seizure(More)
Many endogenous neurochemicals that are known to have important functions in the mature central nervous system have also been found in the developing human cerebellum. Cholinergic neurons, as revealed by immunoreactivities towards choline acetyltransferase or acetylcholinesterase, appear early at 23 weeks of gestation in the cerebellar cortex and deep(More)
1. The responses of periphery (PNS) and central nervous systems (CNS) towards nerve injury are different: while injured mammalian periphery nerons can successfully undergo regeneration, axons in the central nervous system are usually not able to regenerate. 2. In the present study, the genes which were differentially expressed in the PNS and CNS following(More)
Urocortin3 (Ucn3) is an endogenous ligand for corticotropin-releasing factor receptor subtype 2 (CRF2R). In this study, we examined its potential cardiovascular effects by microinjection of Ucn3 and anti-sauvagine 30 (ASV30), a selective antagonist of CRF2R, into the paraventricular nucleus (PVN) of the hypothalamus. After Ucn3 (10 pmol/100 nl) was(More)
OBJECTIVE To analyze the relationship between the proto-oncogene c-fos change and neuronal degeneration in the hippocampus of the patients with Alzheimer's disease. METHODS The post mortem human hippocampal tissues were divided into three groups, namely, the aged, the young and Alzheimer's disease (AD) groups. Each group consisted of 10 patients. The(More)
OBJECTIVE To understand the cellular genetic expression of the cell's population by studying the nucleolar organizer regions (NORs) of hippocampal neuron of Alzheimer's disease (AD). METHODS The postmortem human hippocampal tissues were divided into three groups, namely, the young, the elderly and the AD groups. Each group contained tissues from 10(More)
Epilepsy threatens the health of more than 50 million people all over the world. The temporal lobe epilepsy (TLE) is one of the most common forms of epilepsy and still is one of the commonest drug-resistant epilepsies (so called refractory epilepsy). Vagus nerve stimulation (VNS) was the first non-pharmaceutical therapy used for the treatment of medically(More)
After crushing the right sciatic nerve of rat, the contents of both tubulin and beta tubulin mRNA increased significantly in the ventral horns of spinal cord. Analysis of tubulin in bilateral ventral horns by SDS-PAGE showed that tubulin in the injured side increased by 17%-121% of the control side. The results of in situ hybridization indicated with the(More)
The effects of rhCNTF on dorsal root ganglia (DRG) sensory neurons and spinal cord (SC) motor neurons of 10-day chicken embryos were observed with serum-free culture. The results indicated that rhCNTF supported the survival and differentiation of these neurons and showed certain dose-dependent manners. No effect was found at 0.5 ng/ml rhCNTF concentration,(More)